Publications by authors named "Amanda Mengotto"
Introduction: Cognitive activity and awareness during cardiac arrest (CA) are reported but ill understood. This first of a kind study examined consciousness and its underlying electrocortical biomarkers during cardiopulmonary resuscitation (CPR).
Methods: In a prospective 25-site in-hospital study, we incorporated a) independent audiovisual testing of awareness, including explicit and implicit learning using a computer and headphones, with b) continuous real-time electroencephalography(EEG) and cerebral oxygenation(rSO) monitoring into CPR during in-hospital CA (IHCA).
Introduction: Opioids are often used to manage postoperative pain. Non-narcotic alternatives have increasingly been used to reduce opioid usage. We conducted an open-label randomized non-inferiority clinical trial to compare non-opioid to opioid therapy for pain management after nephrolithiasis surgery.
View Article and Find Full Text PDFMost known SARS-CoV-2 neutralizing antibodies (nAbs), including those approved by the FDA for emergency use, inhibit viral infection by targeting the receptor-binding domain (RBD) of the spike (S) protein. Variants of concern (VOC) carrying mutations in the RBD or other regions of S reduce the effectiveness of many nAbs and vaccines by evading neutralization. Therefore, therapies that are less susceptible to resistance are urgently needed.
View Article and Find Full Text PDFAim: To describe the impact of extracorporeal membrane oxygenation (ECMO) assisted CPR (E-CPR) on cerebral oxygen delivery during in-hospital cardiac arrest (IHCA).
Methods: Retrospective case series from a tertiary academic medical center. Regional cerebral oxygen saturation (rSO2) was measured continuously using cerebral oximetry in six patients who experienced IHCA.
The coronavirus disease 2019 (COVID-19) global pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to place an immense burden on societies and health care systems. A key component of COVID-19 control efforts is serological testing to determine the community prevalence of SARS-CoV-2 exposure and quantify individual immune responses to prior SARS-CoV-2 infection or vaccination. Here, we describe a laboratory-developed antibody test that uses readily available research-grade reagents to detect SARS-CoV-2 exposure in patient blood samples with high sensitivity and specificity.
View Article and Find Full Text PDFArticle Synopsis
- COVID-19, caused by the SARS-CoV-2 virus, has created a significant demand for high-quality Spike (S) protein for research and clinical use.
- *The study evaluates the expression and purification of S protein using Expi293F and ExpiCHO-S cell lines, with findings showing that ExpiCHO-S cells yield better quality and quantity of S proteins.
- *Research confirms the proteins have proper biochemical properties and antigenicity, while also revealing no new binding partners for the Spike protein in human cells, contributing to the understanding of SARS-CoV-2 for various applications.
Comparing methods of performing geographically targeted rural health surveillance.
Emerg Themes Epidemiol
November 2020
Background: Worsening socioeconomic conditions in rural America have been fueling increases in chronic disease and poor health. The goal of this study was to identify cost-effective methods of deploying geographically targeted health surveys in rural areas, which often have limited resources. These health surveys were administered in New York's rural Sullivan County, which has some of the poorest health outcomes in the entire state.
View Article and Find Full Text PDFArticle Synopsis
- The COVID-19 pandemic, caused by SARS-CoV-2, exerts significant pressure on global healthcare systems, making serologic testing crucial for understanding community exposure and immune response.
- * A new laboratory-developed antibody test has been introduced that uses common research-grade materials to effectively detect past SARS-CoV-2 exposure in blood samples, demonstrating high sensitivity and specificity.
- * This test can also measure specific IgG titers from a single sample, making it a practical and cost-efficient tool for evaluating individual immune responses in the context of the ongoing pandemic.
Article Synopsis
- - There is a critical demand for vaccines and treatments to combat COVID-19, which relies on effective testing methods to assess immune responses and identify promising antiviral candidates.
- - Researchers have created a new virus strain called rVSV-SARS-CoV-2 S that mimics the entry characteristics of SARS-CoV-2, allowing for better assessment of immune responses against it.
- - The study demonstrates that this recombinant virus can be used in high-throughput assays to evaluate neutralizing antibodies from recovered COVID-19 patients, paving the way for targeted therapies and deeper understanding of how the virus enters cells.
Article Synopsis
- The study focuses on the urgent need for high-quality SARS-CoV-2 Spike protein to aid in research and vaccine development amid the COVID-19 pandemic.
- Researchers evaluated the expression of Spike protein in two cell lines (Expi293F and ExpiCHO-S), finding that ExpiCHO-S cells produced better yields of the protein.
- The produced S proteins were characterized to ensure their quality, demonstrating consistent behavior in serology tests and effective binding to host cells, supporting various studies to combat the pandemic.
Article Synopsis
- There is a critical need for effective vaccines and treatments for COVID-19, which relies on developing reliable viral assays to evaluate immune responses and select antiviral candidates.
- The study introduces a recombinant vesicular stomatitis virus (rVSV) that incorporates the SARS-CoV-2 spike protein, mimicking the virus's entry behavior for research purposes.
- The findings demonstrate that this rVSV can effectively assess the neutralizing antibodies in COVID-19 recovered patients, indicating its potential for developing targeted vaccines and therapies.