Treatment of obstructive sleep apnea alters cancer-associated transcriptional signatures in circulating leukocytes.

Rationale: Obstructive sleep apnea (OSA) has been associated with a number of chronic disorders that may improve with effective therapy. However, the molecular pathways affected by continuous positive airway pressure (CPAP) treatment are largely unknown. We sought to assess the system-wide consequences of CPAP therapy by transcriptionally profiling peripheral blood leukocytes (PBLs).

A pathway-based analysis on the effects of obstructive sleep apnea in modulating visceral fat transcriptome.

Rationale: Obstructive sleep apnea (OSA) has been associated with metabolic dysregulation and systemic inflammation. This may be due to pathophysiologic effects of OSA on visceral adipose tissue. We sought to assess the transcriptional consequences of OSA on adipocytes by utilizing pathway-focused analyses.

Clinical presentation of shift workers to a sleep clinic.

Purpose: The purpose of this study was to assess the relationship between shift work (SW) history and symptom severity in a sleep clinic population.

Methods: A retrospective chart review of 1,275 employed adult patients referred to a sleep disorders clinic was performed. Patients were categorized as working day shift, fixed evening or night shift, or rotating shifts.

Sleep duration and circulating adipokine levels.

Introduction: Short sleep duration is associated with systemic inflammation and diabetes; however the mechanisms by which reduced sleep leads to these complications are unclear. One possibility is sleep may impact secretion of adipocyte derived hormones that regulate inflammation and insulin resistance. In this study we assessed the association between sleep duration and 3 adipokine levels.

The Epworth score in African American populations.

Introduction: African Americans have elevated scores on the Epworth Sleepiness Scale (ESS) compared to whites. The reason for this difference is not clear.

Methods: Responses to the ESS were assessed in 687 patients (52.

Evidence that rheumatoid arthritis synovial T cells are similar to cytokine-activated T cells: involvement of phosphatidylinositol 3-kinase and nuclear factor kappaB pathways in tumor necrosis factor alpha production in rheumatoid arthritis.

Objective: To investigate the mechanism that leads to the spontaneous production of tumor necrosis factor alpha (TNFalpha) in rheumatoid arthritis (RA) synovial tissue.

Methods: Normal blood monocytes were cocultured either with fixed activated T cells generated from normal blood or RA synovial T cells purified from synovium. TNFalpha production was measured in supernatants from these cocultures following blockade of the transcription factor nuclear factor kappaB (NF-kappaB) using adenoviral transfer of the inhibitor of NF-kappaB kinase alpha into the responding monocytes, or blockade of phosphatidylinositol 3-kinase (PI 3-kinase) using the inhibitory drugs wortmannin or LY294002.