Publications by authors named "Amanda K Staples"
Background: Whole genome sequencing promises to revolutionize our ability to link genotypic and phenotypic variation in a wide range of model and non-model species.
Results: Here we describe the isolation and characterization of a novel mycobacteriophage named BGlluviae that grows on Mycobacterium smegmatis mc155. BGlluviae normally produces turbid plaques but a spontaneous clear plaque was also recovered.
Article Synopsis
- Researchers sequenced the complete genomes of 44 phages that infect the bacterial strain ATCC 21022.
- The phages have double-stranded DNA genomes, varying in size from 15,680 to 70,707 base pairs, and their G+C contents range from 45.1% to 68.5%.
- The study includes phages with three different tail types, classified into specific families.
Article Synopsis
- - Three bacteriophages named AlleyCat, Edugator, and Guillsminger were isolated from enriched soil samples using a specific medium (mc155).
- - All three belong to the same mycobacteriophage subcluster K5 and have genomes ranging from 62,112 to 63,344 base pairs.
- - Each phage has between 92 to 99 predicted protein-coding genes and one tRNA, with Guillsminger being unique as the first mycobacteriophage found to carry an IS family transposon.
The vast bacteriophage population harbors an immense reservoir of genetic information. Almost 2000 phage genomes have been sequenced from phages infecting hosts in the phylum Actinobacteria, and analysis of these genomes reveals substantial diversity, pervasive mosaicism, and novel mechanisms for phage replication and lysogeny. Here, we describe the isolation and genomic characterization of 46 phages from environmental samples at various geographic locations in the U.
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