Extending culture time to improve Mitotic Index for cytogenetic dosimetry.

Purpose: To analyze the effects of extending lymphocyte cultivation time on the Mitotic Index, frequency of first-division cells, and dose estimation after irradiating blood samples with different doses of radiation.

Materials And Methods: Blood samples from two healthy male volunteers were separately irradiated with three doses (3, 5, and 6 Gy) using a Co gamma source (average dose rate: 1.48 kGy.