Regulatory T cells mediate specific suppression by depleting peptide-MHC class II from dendritic cells.

Regulatory T cells (T cells) can activate multiple suppressive mechanisms in vitro after activation via the T cell antigen receptor, resulting in antigen-independent suppression. However, it remains unclear whether similar pathways operate in vivo. Here we found that antigen-specific T cells activated by dendritic cells (DCs) pulsed with two antigens suppressed conventional naive T cells (T cells) specific for both cognate antigens and non-cognate antigens in vitro but suppressed only T cells specific for cognate antigen in vivo.

Ex-vivo iTreg differentiation revisited: Convenient alternatives to existing strategies.

Ex-vivo differentiation of regulatory T cells (Tregs) from naïve CD4 T-cells has been widely used in immunological research. Isolation of a highly pure naïve T cell population is the key factor that determines the efficiency of subsequent Treg differentiation. Currently, this step relies mostly on FACS sorting, which is often costly, time consuming, and inconvenient.

A Simple, Versatile Antibody-Based Barcoding Method for Flow Cytometry.

Barcoding of biological samples is a commonly used strategy to mark or identify individuals within a complex mixture. However, cell barcoding has not yet found wide use in flow cytometry that would benefit greatly from the ability to analyze pooled experimental samples simultaneously. This is due, in part, to technical and practical limitations of current fluorescent dye-based methods.