Publications by authors named "Amanda Christiano"
High apolipoprotein B-containing (apoB-containing) low-density lipoproteins (LDLs) and low apoA1-containing high-density lipoproteins (HDLs) are associated with atherosclerotic cardiovascular diseases. In search of a molecular regulator that could simultaneously and reciprocally control both LDL and HDL levels, we screened a microRNA (miR) library using human hepatoma Huh-7 cells. We identified miR-541-3p that both significantly decreases apoB and increases apoA1 expression by inducing mRNA degradation of 2 different transcription factors, Znf101 and Casz1.
View Article and Find Full Text PDFArticle Synopsis
- High levels of apoB in LDL and low levels of apoA1 in HDL contribute to atherosclerosis, prompting researchers to find a molecular regulator that can balance both lipoproteins.
- In a study using human liver cells, miR-541-3p was identified as a key regulator that reduces apoB while increasing apoA1, by degrading specific transcription factors (Znf101 and Casz1) that control their expression.
- Experiments in mice showed that knocking down related genes altered lipoprotein levels and decreased atherosclerosis without causing fat accumulation in the liver, indicating that miR-541-3p and its targets could be potential therapies for cardiovascular diseases.
Background: The triglyceride (TG) transfer activity of microsomal triglyceride transfer protein (MTP) is essential for lipoprotein assembly in the liver and intestine; however, its function in adipose tissue, which does not assemble lipoproteins, is unknown. Here we have elucidated the function of MTP in adipocytes.
Approach And Results: We demonstrated that MTP is present on lipid droplets in human adipocytes.