Publications by authors named "Aman D Moudgil"

Anaplasma marginale, a tick-borne obligate intracellular rickettsia is incriminated to cause heavy economic losses throughout the tropical and subtropical regions, including India. However, studies highlighting the phylogeography and demographic dynamics of A. marginale are very scant from India.

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  • A genetic tree revealed a major clade (BvG1) with a separate Taiwanese isolate identified as a new genotype (BvG2), showing some genetic variation between them (92.5-93.8% nucleotide identity).
  • The study found high haplotype diversity among B. vogeli populations, particularly in India, with geographical structuring observed, where most haplotypes were country-specific, indicating limited genetic sharing between regions.
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  • The study focused on the genetic analysis of the fish species B. vogeli using cytochrome b gene sequences to understand its population structure.
  • Phylogenetic analysis revealed that all B. vogeli sequences belong to a single group but are split into two subclades (Bv1 and Bv2), with several nucleotide variations leading to amino acid changes.
  • The study found low genetic diversity within B. vogeli populations, confirmed a geographical distinction between Indian and Chinese populations, and suggested a stable population size based on neutrality tests.
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  • Bovicola caprae, an ectoparasite affecting goats globally, was studied in India to confirm its molecular characteristics and analyze its genetic diversity.
  • The study identified two distinct lineages of B. caprae, with Indian sequences clustering with archived sequences from China and Iran.
  • Results showed low nucleotide diversity but high haplotype diversity, indicating recent population expansion, along with significant genetic differentiation and limited gene flow among populations.
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The present study aimed to analyze the cladistics and population structure analysis of Rhipicephalus microplus ticks infesting buffaloes in Haryana, India, as well as the assessment of the anti-tick efficacy of the ethanolic extracts of Cassia fistula (bark, pod pulp, and flowers) against R. microplus larvae. The molecular characterization and population structure analysis were performed by targeting the amplification of the partial mitochondrial cytochrome C oxidase subunit 1 (cox1) gene, whereas anti-tick efficacy was evaluated using a larval packet test.

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  • Hyalomma anatolicum is a tick species known for spreading diseases to both animals and humans, and this study focuses on its genetic structure using mitochondrial gene sequences.
  • Analysis of 75 tick sequences revealed 24 different haplotypes, with Haplotype 1 being the most common across various countries, indicating a recent increase in population size.
  • The study's findings suggest low genetic differentiation among populations and highlight the significance of H. anatolicum in disease transmission, reinforcing its status as an economically important species.
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Introduction: Equine theileriosis, an economically important disease that affects horses and other equids worldwide, is caused by a tick-borne intracellular apicomplexan protozoa . Genotyping of based on the 18S rRNA gene revealed the presence of two, three, four or five genotypes. In previous published reports, these genotypes have been labelled either alphabetically or numerically, and there is no uniformity in naming of these genotypes.

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The etiological agents of zoonotic cystic echinococcosis comprise the Echinococcus granulosus sensu lato (s.l.) species complex.

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  • - The study focused on analyzing the genetic diversity of *Theileria annulata* using the cytochrome b gene, examining samples from various countries, including India, to assess population structure and buparvaquone resistance.
  • - A maximum likelihood tree showed all analyzed *T. annulata* sequences grouped in one clade, but most haplotypes were unique to specific countries, indicating significant genetic differentiation with low gene flow between populations.
  • - While all *T. annulata* isolates remained sensitive to buparvaquone, two new mutations in the cyt b gene associated with drug resistance were uncovered for the first time, highlighting potential concerns for treatment efficacy.
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Purpose: Echinococcus granulosus sensu stricto (s. s.) consists of the most widespread genotypes (G1, G3) implicated in human cystic echinococcosis worldwide.

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Rhipicephalus microplus, a hematophagous vector prevalent in the tropics and subtropics, is responsible for huge economic losses throughout the globe. However, the taxonomy of the tick species, especially prevalent in north India and south China has been challenged in the recent past. The present study attempted to assess the cryptic status of R.

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  • Around 100 species of Physaloptera infect various animals, making identification, especially of larvae, challenging based on physical characteristics alone.
  • This study aimed to molecularly identify Physaloptera larval infections in northern palm squirrels, using the nuclear 18S rRNA gene for confirmation and phylogenetic analysis.
  • Histopathological examinations showed structural features of the parasites and highlighted the northern palm squirrel as a likely second intermediate host for P. praeputialis.
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  • The study investigated the genetic diversity of T. annulata using nearly complete 18S rRNA gene sequences from GenBank™, identifying 70 relevant sequences among 312 total entries.
  • A maximum likelihood phylogenetic analysis revealed two main clades; one included true T. annulata sequences while two isolates were closely related to T. lestoquardi.
  • The findings suggest a broader diversity of Theileria species in the region, including T. annulata and T. orientalis, highlighting potential misidentifications in previous studies.
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The present study had two aims: molecular characterization of Hyalomma dromedarii infesting one-humped camels of Haryana (North India), and assessment of the acaricidal potential of herbal methanolic extracts against H. dromedarii larvae in comparison to synthetic acaricides. Phylogenetics and population neutrality indices were assessed by targeting partial amplification of mitochondrial 16S rDNA sequences.

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Purpose: Porcine cysticercosis is a neglected zoonotic disease of significant veterinary and medical importance owing to its economic impact and public health significance. The present study aimed at genetic characterization of Taenia solium metacestodes in slaughtered pigs of Haryana (North India).

Methods: A total of 213 (160 and 53 from Chandigarh and Hisar, respectively) slaughtered pigs intended for human consumption were screened for the presence of T.

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  • * Genetic analysis using mitochondrial gene sequencing showed that Indian isolates are closely related to those from other countries, but one isolate from Himachal Pradesh formed a distinct subgroup.
  • * The research indicated low genetic diversity among metacestODES in North India, suggesting demographic expansion and a lack of genetic differentiation among the populations.
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  • The study investigated the genetic diversity and phylogenetic relationships of Babesia caballi using 92 complete sequences of the V4 hypervariable 18S rRNA gene from GenBank.
  • The phylogenetic analysis identified two main clades (A and B), with clade A further split into subclades A1 and A2, while clade B exhibited higher genetic diversity among its sequences.
  • Significant nucleotide variations and molecular signature residues in the V4 region were noted, with a comprehensive comparison of genetic variations across different countries highlighted.
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Purpose: Hydatid disease is one of the neglected and challenging (for diagnosis as well as for treatment) parasitic diseases. Along with adverse effect on animal's health leading to production losses, hydatidosis is also associated with huge economic losses. The present study was envisaged with an aim to assess the phylogeny and pathological changes due to natural hydatid cysts in lungs and liver of slaughtered buffaloes in north India.

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The purpose of this study was to detect the antibodies against Toxoplasma gondii in Royal Bengal tigers (Panthera tigris tigris), Asiatic lions (Panthera leo persica), leopards (Panthera pardus), and elephants (Elephas maximus indicus) residing in the Mahendra Chaudhury Zoological Park, in Chhatbir, Punjab (India) during winter and monsoon seasons. Using  indirect ELISA, 20 serum samples were analysed during the winter season. Results indicated that 1 lion (5%) tested seropositive, and 3 tigers and 1 lion (20%) were considered suspect.

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Introduction: This study aimed to assess Trichuris species infection and evaluate the anthelmintic efficacy of fenbendazole and ivermectin against natural trichurid infections in non-human primates (NHPs), kept at Mahendra Chaudhury (MC) Zoological Park, Chhatbir, India.

Materials And Methods: Molecular confirmation of Trichuris infection was carried out using polymerase chain reaction targeting internal transcribed spacer sequences, and anthelmintic efficacy was assessed by fecal egg count reduction test, respectively.

Results: A 710 base pair product confirmed Trichuris species infection in NHPs.

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The present study was envisaged with an aim to evaluate gastrointestinal parasitic infections in a herd of conserved Gaddi (goat) breed bucks (6-9 months of age) kept on semi-intensive feeding management. The individuals (n = 20) representing the herd were selected and divided into two groups; group I (n = 10), clinically ill and group II (n = 10), sub clinically infected individuals. The clinical examination revealed anemia, emaciation and rise in body temperature of the individuals of group I as compared to group II.

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The small strongyles also called as cyathostomes, cyathostomins or trichonemes, affect the health status of equines, leading to morbidity and mortality in heavily infested individuals. The present study was carried out with the aim to identify the cause of colic in a mule of Palam valley, Himachal Pradesh, India. The detailed faecal sample examination revealed heavy intensity of strongyle eggs with an egg per gram value of 2300.

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Descriptive morphology of multifocal hepatic cysts found in eight of forty five (17.78 %) Wistar rats sacrificed during pharmacological studies related to herbal formulations was studied. The creamish to white cysts were of varying sizes, ranging from 3-8 mm in diameter.

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A total of 246 faecal/scat samples of the dogs were screened by direct and floatation concentration technique to study the gastrointestinal (GI) tract parasitism in dogs of Palampur, Himachal Pradesh, India. Detailed coprological examination targeting different seasons, age groups and living styles of the dogs revealed an overall 28.04 % of GI parasitism with highest prevalence in summer season (37.

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The detection of Trypanosoma evansi in blood is intricate, primarily in chronic stage of infection, as the parasitaemia is often low and fluctuating. The climatic conditions of the target area of Punjab (a province of India with a total of 34,000 horses and ponies used for sports and transport) are conducive for the parasite propagation. The objective of present investigation was to assess the prevalence of T.

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