Why are they not coming back? A single-center follow-up study on oncological women oocyte's storing for fertility preservation.

Introduction: Oocyte cryopreservation is a valid option for female cancer patients to preserve fertility. The number of patients undergoing fertility preservation (FP) cycles has increased over the past years. Nevertheless, the rates of patients returning to use their cryopreserved material have shown to be considerably low, ranging from 5-8%, but significant data regarding the reasons of such low return rates are scarce.

Inter-centre reliability in embryo grading across several IVF clinics is limited: implications for embryo selection.

Research Question: What is the intra- and inter-centre reliability in embryo grading performed according to the Istanbul Consensus across several IVF clinics?

Design: Forty Day 3 embryos and 40 blastocysts were photographed on three focal planes. Senior and junior embryologists from 65 clinics were invited to grade them according to the Istanbul Consensus (Study Phase I). All participants then attended interactive training where a panel of experts graded the same embryos (Study Phase II).

Preimplantation Genetic Testing for Aneuploidy Improves Clinical, Gestational, and Neonatal Outcomes in Advanced Maternal Age Patients Without Compromising Cumulative Live-Birth Rate.

Purpose: To report the effects of blastocyst stage aneuploidy testing on clinical, gestational, and neonatal outcomes for patients of advanced maternal age undergoing IVF.

Methods: This is a single-center observational-cohort study with 2 years follow-up. The study includes a total of 2538 couples undergoing 2905 egg collections (control group), 308 (PGT-A), and 106 (drop-out group, consenting for PGT-A but withdrawing due to poor embryological outcome) RESULTS: Compared with control group, PGT-A showed improved clinical outcomes (live-birth rate per transferred embryo, LBR 40.

Oocyte Cryopreservation in Oncological Patients: Eighteen Years Experience of a Tertiary Care Referral Center.

The aim of the present study is to report our experience on elective women fertility preservation before cancer treatment. This is a single-center retrospective observational study, including all patients who underwent elective fertility preservation before oncological treatment between January 2001 and March 2019 at our Institute. Of a total of 568 women who received fertility counseling, 244 (42.

Delayed childbearing and female ageing impair assisted reproductive technology outcome in survivors of male haematological cancers.

Purpose: To analyse the impact of female characteristics on assisted reproductive technology outcome among male haematological cancer survivors.

Methods: A retrospective analysis of 93 haematological cancer survivors attending our tertiary referral fertility centre between June 1998 and June 2017 for achieving fatherhood with assisted reproductive technology treatments.

Results: A progressive increase in the median female age was observed during the study period (32.

Human oocyte ultravitrification with a low concentration of cryoprotectants by ultrafast cooling: a new protocol.

Objective: To evaluate the efficacy of a new ultravitrification technique with a low concentration of cryoprotectants.

Design: Ultravitrification research.

Setting: Private assisted reproduction center.

Results of in vitro fertilization in Italy after the introduction of a new law.

Objective: To investigate the consequences of a law introduced in Italy in 2004 that forbids the fertilization or injection of more than three oocytes for assisted reproduction and does not allow any embryo selection or cryopreservation.

Design: Retrospective observational analysis.

Setting: Subfertile patients enrolled in an assisted reproduction program.

Normal birth after transfer of cryopreserved human embryos generated by microinjection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.

Objective: To report the first birth after transfer of cryopreserved embryos generated by intracytoplasmic sperm injection of cryopreserved testicular spermatozoa into cryopreserved human oocytes.

Design: Case report.

Setting: Tertiary center for reproductive technology.

Cryopreservation of a small number of spermatozoa in yolk-filled human zonae pellucidae.

Objectives: Conventional sperm freezing procedures need the addition of a relatively large volume of cryoprotectant. The dilution of extremely poor sperm suspensions from ejaculate or testicular tissue may make the recovery of viable spermatozoa difficult at the moment of the intracytoplasmic sperm injection (ICSI) procedure. The cryopreservation of a few spermatozoa in empty zonae pellucidae is an interesting solution for crypto-azoospermic infertile men.