Heat shock protein expression during stress and diapause in the marine copepod Calanus finmarchicus.

Calanoid copepods, such as Calanus finmarchicus, are a key component of marine food webs. C. finmarchicus undergo a facultative diapause during juvenile development, which profoundly affects their seasonal distribution and availability to their predators.

A transcriptional element of the diapause program in the Asian tiger mosquito, Aedes albopictus, identified by suppressive subtractive hybridization.

Many temperate insects cope with the unfavorable conditions of winter by entering a photoperiodic diapause, but the molecular basis of this crucial adaptation is not well understood. In temperate populations of Aedes albopictus, exposure to short-day lengths causes the adult female to oviposit diapausing eggs. Suppressive subtractive hybridization (SSH) and quantitative reverse transcription PCR (qRT-PCR) were performed on RNA isolated from mature (stage V) oocytes of a temperate population of Ae.