Western blot analysis of Leishmania infantum antigens using sera from pentamidine-treated dogs.

Pentamidine treatment has been used successfully to establish immune cellular responses in recovered dogs. In this paper, we examined the appearance and disappearance of antibodies over time against crude Leishmania antigens and purified gp63 or gp70 proteins in sera from cured dogs using a Western blotting technique. Following the treatment, a pattern of antibody specificities to parasite antigens was observed in the sera of cured dogs.