Publications by authors named "Amaal M Alhelli"

Cheddar cheese proteolysis were accelerated employing Penicillium candidum PCA1/TT031 protease into cheese curd. In the present study, several of the significant factors such as protease purification factor (PF), protease concentration and ripening time were optimized via the response surface methodology (RSM). The ideal accelerated Cheddar cheese environment consisted of 3.

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Tempoyak is a functional Malaysian food (an acid-fermented condiment) which is produced from the pulp of the durian () fruit. The current study aimed to isolate and identify potential exopolysaccharide (EPS)-producing strains from tempoyak for potential use as probiotics. Seven isolates (DUR2, DUR4, DUR5, DUR8, DUR12, DUR18, and DUR20) out of 44 were able to produce EPS, and exhibited resistance to acid and bile salt compared to the reference strains (ATCC53103) and (ATCC8014).

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Article Synopsis
  • PCA 1/TT031 produces various extracellular proteases, and the study aimed to enhance the purification of these enzymes using an aqueous two-phase system (ATPS) combined with Response Surface Methodology (RSM).
  • Optimal conditions for purification were found to be using 9.0% PEG 8000, 5.2% NaCl, and 15.9% sodium citrate, achieving a significant 93% yield and a purification factor of 6.8.
  • Further characterization revealed the enzyme has a molecular weight between 100 and 140 kDa, functions optimally at pH 6.0 and 50°C, and could be beneficial for applications in the dairy industry, particularly in cheese ripening.
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This report shows the partitioning and purification of alkaline extracellular lipase from Penicillium candidum (PCA 1/TT031) by solid-state fermentation (SSF). In the present analysis, some of the important parameters such as PEG concentration, PEG molecular mass, salt concentration and buffer concentration were optimised through the response surface methodology (RSM). The optimum aqueous two-phase systems (ATPS) environment consisted of 13.

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