Effect of Aggregation and Molecular Size on the Ice Nucleation Efficiency of Proteins.

Aerosol acts as ice-nucleating particles (INPs) by catalyzing the formation of ice crystals in clouds at temperatures above the homogeneous nucleation threshold (-38 °C). In this study, we show that the immersion mode ice nucleation efficiency of the environmentally relevant protein, ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO), occurs at temperatures between -6.8 and -31.

Pollen Emissions of Subpollen Particles and Ice Nucleating Particles.

Pollen grains significantly contribute to the aerosol population, and levels are predicted to increase in the future. Under humid atmospheric conditions, pollen grains can rupture creating pollen grain fragments referred to as subpollen particles (SPPs) which are dispersed into the atmosphere with wind. In this laboratory study, SPP emission factors were determined for ryegrass, sp.

Ocean Aerobiology.

Ocean aerobiology is defined here as the study of biological particles of marine origin, including living organisms, present in the atmosphere and their role in ecological, biogeochemical, and climate processes. Hundreds of trillions of microorganisms are exchanged between ocean and atmosphere daily. Within a few days, tropospheric transport potentially disperses microorganisms over continents and between oceans.

Chromophoric dissolved organic matter (CDOM) in a subtropical estuary (Galveston Bay, USA) and the impact of Hurricane Harvey.

The landfall of Hurricane Harvey in August 2017 provided the opportunity to study the impact of extreme freshwater discharge on chromophoric dissolved organic matter (CDOM) properties in a subtropical estuary (Galveston Bay, Texas). Both fluorescence spectroscopy (excitation-emission matrices) and a three-component parallel factor analysis (PARAFAC) model identified changes in CDOM properties. Comparing to Coble's peaks, component 1 was similar to peak C, component 2 to peak M, and component 3 to peak B.

Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host.

Best practices in laboratory culture management often include cryopreservation of microbiota, but this can be challenging with some virus particles. By preserving viral isolates researchers can mitigate genetic drift and laboratory-induced selection, thereby maintaining genetically consistent strains between experiments. To this end, we developed a method to cryopreserve the model, green-alga infecting virus, Paramecium bursaria Chlorella virus 1 (PBCV-1).