Publications by authors named "Alyssa Mitson-Salazar"
Pathogenic Effector Th2 Cells in Allergic Eosinophilic Inflammatory Disease.
Front Med (Lausanne)
October 2017
Article Synopsis
- Th2 cells are essential for the development of eosinophil-driven type 2 inflammation caused by allergens.
- Recent research highlights a subset of Th2 cells, called pathogenic effector Th2 (peTh2) cells, that have stronger disease-causing abilities compared to typical Th2 cells.
- The article reviews the characteristics and functions of peTh2 cells in both animal studies and human cases, focusing on their potential as a target for treating allergic eosinophilic inflammation.
Article Synopsis
- IL-5(+) pathogenic effector T(H)2 cells (peT(H)2) are a specific subpopulation of T(H)2 cells known for their strong pro-inflammatory functions, previously studied mainly in mouse models of allergies.
- The research aimed to identify specific markers for human peT(H)2 cells and to explore their role in allergic eosinophilic disorders, involving patients with eosinophilic gastrointestinal disease and atopic dermatitis.
- Findings revealed that peT(H)2 cells have distinct markers and produce higher levels of cytokines IL-5 and IL-13, and are significantly more abundant in patients with allergic conditions compared to healthy individuals, suggesting their key role in allergic inflammation.
Article Synopsis
- Intracellular cytokine staining (ICCS) is a cutting-edge technique that uses fluorescent antibodies and flow cytometry to study cytokine expression in individual cells.* -
- Recent improvements in flow cytometry allow ICCS to measure multiple cytokines at once, revealing complex cellular responses and enabling simultaneous analysis of other important cellular markers.* -
- The protocol includes steps for stimulating cells in the lab, fixing them for analysis, and staining cell surfaces, making ICCS a powerful method compared to older techniques like ELISPOT and T cell cloning.*
Fanconi anemia (FA) is a rare genetic disorder characterized by bone marrow failure and an increased risk for leukemia and cancer. Fifteen proteins thought to function in the repair of DNA interstrand crosslinks (ICLs) comprise what is known as the FA-BRCA pathway. Activation of this pathway leads to the monoubiquitylation and chromatin localization of FANCD2 and FANCI.
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