The effect of acute exercise on GLUT4 levels in peripheral blood mononuclear cells of sled dogs.

Using sled dogs as exercise model, our objectives of this study were to 1) assess the effects of one acute bout of high-intensity exercise on surface GLUT4 concentrations on easily accessible peripheral blood mononuclear cells (PBMC) and 2) compare our findings with published research on exercise induced GLUT4 in skeletal muscle. During the exercise bout, dogs ran 5 miles at approximately 90% of VO max. PMBC were collected before exercise (baseline), immediately after exercise and after 24h recovery.