Procoagulant phospholipid concentration in canine erythrocyte concentrates stored with or without prestorage leukoreduction.

Objective: To evaluate canine erythrocyte concentrates (ECs) for the presence of procoagulant phospholipid (PPL), determine whether PPL concentration changes during the course of storage of ECs, and ascertain whether prestorage leukoreduction (removal of leukocytes via gravity filtration) reduces the development of PPL.

Sample: 10 whole blood units (420 g each) collected from 10 random-source, clinically normal dogs (1 U/dog).

Procedures: The dogs were randomized to 1 of 2 groups.

Cytokine concentration in stored canine erythrocyte concentrates.

Objective: To evaluate the effect of leukoreduction (LR) as compared to standard nonleukoreduced (NLR) units on cytokine concentrations in canine erythrocyte concentrates during regular storage time.

Design: Randomized, experimental study.

Setting: University teaching hospital.

In vitro hypercoagulability on whole blood thromboelastometry associated with in vivo reduction of circulating red cell mass in dogs.

Background: Previous studies have reported a strong correlation between indicators of red cell mass (RCM) and thromboelastometry results (TEM) in several species, specifically an association of apparent hypercoagulability with decreased RCM.

Objectives: The objectives of the study were to (1) evaluate the effect of decreased circulating RCM on TEM results in dogs, and (2) determine the relative contributions of citrate dilution vs in vivo reduction of RCM to hemostatic potential.

Materials And Methods: Thirteen healthy dogs had one unit of blood removed on day 0.

Microparticles in stored canine RBC concentrates.

Background: Transfusion of RBC concentrates may cause adverse effects in the recipient, particularly when stored > 2 weeks. Prestorage removal of WBCs and platelets (leukoreduction, LR) improves clinical outcome in the human recipient. As blood ages during storage, progressive alterations in the structure and function of the cells occur.

Correlation of hematocrit, platelet concentration, and plasma coagulation factors with results of thromboelastometry in canine whole blood samples.

Objective: To evaluate the components of canine whole blood samples that contribute to results of thromboelastometry (TEM).

Animals: 127 healthy dogs.

Procedures: For each dog, a blood sample was collected from a jugular vein into tubes containing no anticoagulant, EDTA, or citrate anticoagulant.

Clot formation in canine whole blood as measured by rotational thromboelastometry is influenced by sample handling and coagulation activator.

The objective of the present study was to systematically evaluate the impact of methodology on thromboelastometry with canine whole blood. Thromboelastometry was performed on citrated blood using a variety of combinations of clotting activators [ex-tem (tissue factor or TF), in-tem (ellagic acid), diluted TF from Innovin, or Ca (recalcification only)] and storage times. Thromboelastometry was also performed using diluted TF from Innovin on blood collected into a contact inhibitor.