Development of silicone-based phantoms for biomedical optics from 400 to 1550 nm.

This work describes the development of silicone-based evaluation phantoms for biomedical optics in the wavelength range from 400 to 1550 nm. The absorption coefficient and the reduced scattering coefficient were determined using an integrating sphere setup. Zirconium dioxide pigments were used as scatterers and carbon black as absorbers.

Auto Aligning, Error-Compensated Broadband Collimated Transmission Spectroscopy.

Broadband spectral measurements of the ballistic transmission of scattering samples are challenging. The presented work shows an approach that includes a broadband system and an automated adjustment unit for compensation of angular distortions caused by non-plane-parallel samples. The limits of the system in terms of optimal transmission and detected forward scattering influenced by the scattering phase function are investigated.

Impact of Multi-Scattered LiDAR Returns in Fog.

In the context of autonomous driving, the augmentation of existing data through simulations provides an elegant solution to the challenge of capturing the full range of adverse weather conditions in training datasets. However, existing physics-based augmentation models typically rely on single scattering approximations to predict light propagation under unfavorable conditions, such as fog. This can prevent the reproduction of important signal characteristics encountered in a real-world environment.

Optical Goniometer Paired with Digital Monte Carlo Twin to Determine the Optical Properties of Turbid Media.

We present a goniometer designed for capturing spectral and angular-resolved data from scattering and absorbing media. The experimental apparatus is complemented by a comprehensive Monte Carlo simulation, meticulously replicating the radiative transport processes within the instrument's optical components and simulating scattering and absorption across arbitrary volumes. Consequently, we were able to construct a precise digital replica, or "twin", of the experimental setup.

Estimation of Photon Path Length and Penetration Depth in Articular Cartilage Zonal Architecture Over the Therapeutic Window.

Objective: The key characteristics of light propagation are the average penetration depth, average maximum penetration depth, average maximum lateral spread, and average path length of photons. These parameters depend on tissue optical properties and, thus, on the pathological state of the tissue. Hence, they could provide diagnostic information on tissue integrity.

Broadband scattering properties of articular cartilage zones and their relationship with the heterogenous structure of articular cartilage extracellular matrix.

Significance: Articular cartilage exhibits a zonal architecture, comprising three distinct zones: superficial, middle, and deep. Collagen fibers, being the main solid constituent of articular cartilage, exhibit unique angular and size distribution in articular cartilage zones. There is a gap in knowledge on how the unique properties of collagen fibers across articular cartilage zones affect the scattering properties of the tissue.

Focusing Coherent Light through Volume Scattering Phantoms via Wavefront Shaping.

Manipulating the wavefront of coherent light incident on scattering media to enhance the imaging depth, sensitivity, and resolution is a common technique in biomedical applications. Local phase variations cause changes in the interference and can be used to create a focus inside or behind a scattering medium. We use wavefront shaping (WFS) to force constructive interference at an arbitrary location.

Generic and Model-Based Calibration Method for Spatial Frequency Domain Imaging with Parameterized Frequency and Intensity Correction.

Spatial frequency domain imaging (SFDI) is well established in biology and medicine for non-contact, wide-field imaging of optical properties and 3D topography. Especially for turbid media with displaced, tilted or irregularly shaped surfaces, the reliable quantitative measurement of diffuse reflectance requires efficient calibration and correction methods. In this work, we present the implementation of a generic and hardware independent calibration routine for SFDI setups based on the so-called pinhole camera model for both projection and detection.

Impact of experimental setup parameters on the measurement of articular cartilage optical properties in the visible and short near-infrared spectral bands.

There is increasing research on the potential application of diffuse optical spectroscopy and hyperspectral imaging for characterizing the health of the connective tissues, such as articular cartilage, during joint surgery. These optical techniques facilitate the rapid and objective diagnostic assessment of the tissue, thus providing unprecedented information toward optimal treatment strategy. Adaption of optical techniques for diagnostic assessment of musculoskeletal disorders, including osteoarthritis, requires precise determination of the optical properties of connective tissues such as articular cartilage.

Confocal Laser Scanning Microscope Imaging of Custom-Made Multi-Cylinder Phantoms: Theory and Experiment.

In this work, the image formation in a confocal laser scanning microscope (CLSM) is investigated for custom-made multi-cylinder phantoms. The cylinder structures were fabricated using 3D direct laser writing and consist of parallel cylinders with radii of 5 and 10 μm for the respective multi-cylinder phantom, with overall dimensions of about 200×200×200 μm3. Measurements were performed for different refractive index differences and by varying other parameters of the measurement system, such as pinhole size or numerical aperture (NA).

Radiative transfer equation-based color prediction and color adjustment strategies.

This paper discusses different strategies for color prediction and matching. Although many groups use the two-flux model (i.e.

Improved topographic reconstruction of turbid media in the spatial frequency domain including the determination of the reduced scattering and absorption coefficients.

The separation of scattering and absorption is of great importance for studying the radiative transfer in turbid media. Obtaining the corresponding coefficients for non-flat objects is difficult and needs special consideration. Building on our previous work [J.

Continuous Sizing and Identification of Microplastics in Water.

The pollution of the environment with microplastics in general, and in particular, the contamination of our drinking water and other food items, has increasingly become the focus of public attention in recent years. In order to better understand the entry pathways into the human food chain and thus prevent them if possible, a precise characterization of the particles concerning their size and material is indispensable. Particularly small plastic particles pose a special challenge since their material can only be determined by means of large experimental effort.

Single scattering models for radiative transfer of isotropic and cone-shaped light sources in fog.

The simulation of rare edge cases such as adverse weather conditions is the enabler for the deployment of the next generation of autonomous drones and vehicles into conditions where human operation is error-prone. Therefore, such settings must be simulated as accurately as possible and be computationally efficient, so to allow the training of deep learning algorithms for scene understanding, which require large-scale datasets disallowing extensive Monte Carlo simulations. One computationally-expensive step is the simulation of light sources in scattering media, which can be tackled by the radiative transfer equation and approximated by analytical solutions in the following.

Analytical solution of the vector radiative transfer equation for single scattered radiance: erratum.

We have discovered a small error in our recently published work [J. Opt. Soc.

Numerical simulation of phase-optimized light beams in two-dimensional scattering media.

Manipulating the incident wavefront in biomedical applications to enhance the penetration depth and energy delivery in scattering media such as biological tissue has gained a lot of attention in recent years. However, focusing inside scattering media and examining the electromagnetic field inside the medium still is an elaborate task. This is where electromagnetic field simulations that model the wavefront shaping process can help us understand how the focal near field evolves at different depths.

Analytical solution of the vector radiative transfer equation for single scattered radiance.

In this paper, derivation of the analytical solution of the vector radiative transfer equation for the single scattered radiance of three-dimensional semi-infinite media with a refractive index mismatch at the boundary is presented. In particular, the solution is obtained in the spatial domain and spatial frequency domain. Besides the general derivation, determination of the amplitude scattering matrix, which is required for the analytical solution, is given in detail.

Spatially resolved reflectance from turbid media having a rough surface. Part I: simulations.

Determining the optical properties of turbid media with spatially resolved reflectance measurements is a well-known method in optical metrology. Typically, the surfaces of the investigated materials are assumed to be perfectly smooth. In most realistic cases, though, the surface has a rough topography and scatters light.

Distance insensitive reflectance setup for the spectrally resolved determination of the optical properties of highly turbid media.

A measurement system for a distance insensitive acquisition of the reflectance from turbid media is presented. The geometric relationships of the detection unit are discussed theoretically and subsequently verified using Monte Carlo simulations. In addition, an experimental setup is presented to prove the theoretical considerations and simulations.

Spatially resolved reflectance from turbid media having a rough surface. Part II: experiments.

Spatially resolved reflectance measurements are a standard tool for determining the absorption and scattering properties of turbid media such as biological tissue. However, in literature, it was shown that these measurements are subject to errors when a possible rough surface between the turbid medium and the surrounding is not accounted for. We evaluated these errors by comparing the spatially resolved reflectance measured on rough epoxy-based samples with Monte Carlo simulations using Lambertian surface scattering, the Cook-Torrance model, and the generalized Harvey-Shack model as surface scattering models.

Imaging of custom-made single scatterers with the confocal laser scanning microscope.

In this work, we investigate image formation in the confocal laser scanning microscope for different single scatterers, both theoretically and experimentally. For spherical scatterers, an effective and fast algorithm was implemented to calculate the confocal image for different diameters and wavelengths. Measurements on a polystyrene sphere (PS) with a diameter of 20 µm confirmed the expected effects, for example, the appearance of a central signal similar to the point spread function of the optical system.

Improved topography reconstruction of volume scattering objects using structured light.

The use of structured light projection enables the reconstruction of three-dimensional topography of surface reflecting objects. However, if the investigated object exhibits volume scattering, the obtained topography is erroneously caused by light undergoing volume scattering inside the object. In this theoretical study, we investigate these errors using Monte Carlo simulations.

Determination of the spectrally resolved extinction coefficient of human dental enamel using collimated transmission spectroscopy.

Objectives: The determination of the extinction coefficient of human dental enamel to deliver further optical properties of enamel to improve the understanding of light propagation in teeth and to improve restoration materials.

Methods: The extinction coefficient was measured within a spectral range of 300-980 nm using a collimated transmission setup. 35 specimens from 16 donors were examined.

Analytical solution of the radiative transfer theory for the coherent backscattering from two-dimensional semi-infinite media.

An analytical solution for coherent backscattering (CBS) in two dimensions was derived by solving the radiative transfer equation. Particularly, the single scattered radiance from a semi-infinite medium containing perpendicularly illuminated cylinders was obtained. At the boundary, a refractive index mismatch was taken into account.

Influence of Lambertian surface scattering on the spatially resolved reflectance from turbid media: a computational study.

The determination of the optical properties in turbid media plays an essential role in medical diagnostics and process control. The method of spatially resolved reflectance measurements is a frequently used tool to evaluate the reduced scattering coefficient as well as the absorption coefficient. In most cases a smooth interface is assumed between the medium under investigation and the surrounding medium.