LC-MS analysis of Hep-2 and Hek-293 cell lines treated with Brazilian red propolis reveals differences in protein expression.

Objective: Red propolis, an exclusive variety of propolis found in the northeast of Brazil has shown to present antitumour activity, among several other biological properties. This article aimed to help to evaluate the underlying molecular mechanisms of the potential anticancer effects of red propolis on tumour, Hep-2, and non-tumour cells, Hek-293.

Methods: Differentially expressed proteins in human cell lines were identified through label-free quantitative MS-based proteomic platform, and cells were stained with Giemsa to show morphological changes.

STUDIES ON A STRAIN OF CHLOROQUINE-RESISTANT PLASMODIUM FALCIPARUM FROM VIET-NAM.

This report presents the results of chemotherapeutic studies carried out with non-immune volunteers infected with a strain of Plasmodium falciparum from Viet-Nam, termed the Viet-Nam (Sn.) strain, under conditions of study precluding reinfection. Nine volunteers received, during acute clinical attacks, three-day courses of chloroquine, seven receiving 1500 mg of chloroquine base orally, one 3000 mg of chloroquine base orally, and one 2160 mg of chloroquine base intramuscularly.

STUDIES ON A STRAIN OF CHLOROQUINE-RESISTANT PLASMODIUM FALCIPARUM FROM THAILAND.

Infections with a strain of Plasmodium falciparum from Thailand, termed the Thailand (JHK) strain, were established in 25 non-immune volunteers in a non-endemic area under conditions precluding reinfection. Eleven volunteers received chloroquine in usually curative doses on a three-day schedule during acute clinical malaria attacks. Volunteers also received (again during acute clinical attacks) hydroxychloroquine, amodiaquine, mepacrine, pyrimethamine, proguanil or 377-C-54, alone or in combination.

Methaemoglobin reduction test: a new, simple, in vitro test for identifying primaquine-sensitivity.

The 8-aminoquinolines, and many other drugs, cause an acute intravascular haemolysis, known as primaquine-sensitivity, in a certain percentage of persons, particularly the darker-skinned peoples of the world. Massive drug programmes for the eradication of malaria in whole population groups frequently call for the use of primaquine; in addition, the use of other haemolytic or potentially haemolytic drugs in clinical medicine is widespread. Thus it is becoming increasingly important to be able to identify primaquine-sensitive individuals in field and clinical laboratories.

Mitigation of the haemolytic effect of primaquine and enhancement of its action against exoerythrocytic forms of the Chesson strain of Piasmodium vivax by intermittent regimens of drug administration: a preliminary report.

Primaquine-an 8-aminoquinoline derivative-is one of the most effective drugs for use against the tissue stages of the malaria parasite. Unfortunately certain persons suffer from an inherited defect of metabolism which renders them susceptible to haemolysis after ingestion of the 8-aminoquinolines, certain other drugs and some vegetables. Susceptibility appears to be inherited by a partially dominant sex-linked gene of variable expression.