Publications by authors named "Alvaro Salles Filho"
The cotton boll weevil (CBW) () is one of the major insect pests of cotton in Brazil. Currently, CBW control is mainly achieved by insecticide application, which is costly and insufficient to ensure effective crop protection. RNA interference (RNAi) has been used in gene function analysis and the development of insect control methods.
View Article and Find Full Text PDFArticle Synopsis
- The study presents a combined method of Agrobacterium and biolistics for transforming cotton into genetically modified (GM) varieties, addressing issues like pest losses and abiotic stresses.
- The protocol involves several steps, including Agrobacterium preparation and embryo co-culture, and results in a high average transformation efficiency of 60% over a period of about 4-10 weeks.
- The transgenic plants exhibit stable inheritance of the transgene, express it at high levels, and show no significant differences in traits or productivity compared to non-GM cotton plants.