Production of carcinoembryonic antigen from a human colon adenocarcinoma cell line. I. Large-scale cultivation of carcinoembryonic antigen-producing cells on cylindric cellulose-based microcarriers.

A continuous cell line from a human colon carcinoma, designated HuCC1-14, was successfully grown on cyclindrical cellulose microcarriers (MC) charged by DEAE. The cells grow on these carriers as large cell-MC aggregates and maintain high cell densities in submerged conditions for extended periods. HuCC1-14 cells secrete into the culture medium significant amounts of carcinoembryonic antigen (CEA).

Production of carcinoembryonic antigen from a human colon adenocarcinoma cell line. II. Use of monoclonal antibodies to carcinoembryonic antigen for antigen purification and characterization.

Monoclonal antibodies (McAbs) were prepared against carcinoembryonic antigen (CEA), derived from human metastases to the liver of primary colon adenocarcinoma. A stable clone, secreting monoclonal anti-CEA antibody (Ab), was selected. The hybridoma cells were grown in BALB/C mice, forming ascites fluids containing 2-5 mg/ml of the relevant McAbs.