Molecular characterization of Morogoro maize-associated virus, a nucleorhabdovirus detected in maize (Zea mays) in Tanzania.

RNAtag-seq of maize samples collected in Tanzania revealed the presence of a previously undescribed nucleorhabdovirus, tentatively named "Morogoro maize-associated virus" (MMaV), in three samples. The MMaV genome is 12,185-12,187 nucleotides long and shares a 69-70% nucleotide sequence identity with taro vein chlorosis virus. Annotation of the genomes showed a typical nucleorhabdovirus gene organization.

Diversity and distribution of Maize-associated totivirus strains from Tanzania.

Typically associated with fungal species, members of the viral family Totiviridae have recently been shown to be associated with plants, including important crop species, such as Carica papaya (papaya) and Zea mays (maize). Maize-associated totivirus (MATV) was first described in China and more recently in Ecuador, where it has been found to co-occur with other viruses known to elicit maize lethal necrosis disease (MLND). In a survey for maize-associated viruses, 35 samples were selected for Illumina HiSeq sequencing, from the Tanzanian maize producing regions of Mara, Arusha, Manyara, Kilimanjaro, Morogoro and Pwani.

Grain-yield stability among tropical maize hybrids derived from doubled-haploid inbred lines under random drought stress and optimum moisture conditions.

Drought is a devastating environmental stress in agriculture and hence a common target of plant breeding. A review of breeding progress on drought tolerance shows that, to a certain extent, selection for high yield in stress-free conditions indirectly improves yield in water-limiting conditions. The objectives of this study were to () assess the genotype × environment (GE) interaction for grain yield (GY) and other agronomic traits for maize ( L.

Unravelling the genetic diversity of the three main viruses involved in Sweet Potato Virus Disease (SPVD), and its practical implications.

SUMMARY Sweetpotato (Ipomoea batatas) is a widely grown food crop, in which the most important diseases are caused by viruses. Genetic variability of three widely distributed sweetpotato viruses was analysed using data from 46 isolates of Sweet potato feathery mottle virus (SPFMV), 16 isolates of Sweet potato mild mottle virus (SPMMV) and 25 isolates of Sweet potato chlorotic stunt virus (SPCSV), of which 19, seven and six isolates, respectively, are newly characterized. Division of SPFMV into four genetic groups (strains) according to phylogenetic analysis of coat protein (CP) encoding sequences revealed that strain EA contained the East African isolates of SPFMV but none from elsewhere.

Incidence of Viruses Infecting Sweetpotato in Tanzania.

A survey for incidence of sweetpotato viruses was carried out in the three sweetpotato-producing districts (Bukoba, Misungwi, and Tarime) in the Lake Victoria basin and in the Indian Ocean coastal zone (Bagamoya on the mainland and Unguja on Zanzibar Island) in Tanzania in March and April 2003. A total of 170 plants from 43 sweetpotato fields were collected, established in an insect-proof screenhouse, and tested for viruses by nitrocellulose membrane enzyme-linked immunosorbent assay (NCM-ELISA). Scions from symptomless plants were grafted onto Ipomoea setosa, a nearly universal indicator plant for sweetpotato viruses, and leaves were tested by NCM-ELISA.