Background: Using dried blood spots (DBS) for quantitation of the antiviral drug oseltamivir (Tamiflu(®)), an ester prodrug, and its active metabolite oseltamivir carboxylate could provide ethical and logistic benefits. Hence, its feasibility was investigated using a previously developed column-switching LC-MS/MS method.
Results: Sensitivity, precision and accuracy in DBS were comparable to standard plasma assays.
J Chromatogr B Analyt Technol Biomed Life Sci
February 2011
Background: A thin-layer chromatography-mass spectrometry interface was previously reported to extract compounds from dried blood spots online. Here, we integrated this interface into a column-switching LC-MS/MS system for online solid-phase extraction and addressed internal standard addition.
Results: A drug was analyzed in dried blood spots with a lower limit of quantitation of 50 pg/ml.
Background: Analysis of mycophenolic acid (MPA), the active form of the immunosuppressive drug mycophenolate mofetil, and its glucuronide metabolite MPAG is required for therapeutic monitoring and postmarketing clinical studies. Dried blood spots (DBS) and dried plasma spots (DPS) could be alternatives to conventional assays for small-volume sampling and easy shipment.
Results: A LC-MS/MS method with online SPE was established using stable isotope labeled analytes as internal standards.