Publications by authors named "Alma I Santos-Diaz"
Background: Despite the agreed principle that access to food is a human right, undernourishment and metal ion deficiencies are public health problems worldwide, exacerbated in impoverished or war-affected areas. It is known that maternal malnutrition causes growth retardation and affects behavioral and cognitive development of the newborn. Here we ask whether severe caloric restriction leads per se to disrupted metal accumulation in different organs of the Wistar rat.
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- Immunostaining is a crucial technique for pinpointing protein locations in cells and tissues by using antibodies with various markers, mainly in immunofluorescence for research and diagnosis.
- The technique has its challenges, such as fixing samples and managing antibody incubation times, which necessitate continual improvements in established protocols.
- This review offers optimized methods for immunofluorescence and introduces a new antibody signal enhancer that boosts detection signals, particularly in cervical cancer and fibrous tissues, making it useful for both seasoned and novice researchers.