Total and Chemoenzymatic Synthesis of Seongsanamide E.

The total and chemoenzymatic synthesis of the depsipeptide natural product seongsanamide E, , is described. The synthetic C-terminal -acetylcysteamine thioester of linear natural product was macrolactonized by the excised recombinant purified seongsanamide thioesterase (Sgd-TE) domain, generating . Sgd-TE also effects the ring opening of .