[Construction of libraries of structural genes (cDNA) and "jumping" gene libraries in lambda vectors].

Main approaches and methods used for constructing "jamping" and cDNA libraries, including novel ones, that omit the employment of methylases and linkers, are presented. The advantages and drawbacks of the well-known and new lambda vectors, suitable for the purposes mentioned, are discussed. Special attention is paid to diphasmids lambda ZAP, lambda SK12, lambda SK15, that combine features of lambda and M13 phages and of plasmids.

[Nucleotide sequence of the rplL gene coding for ribosomal protein L7/L12 of Pseudomonas putida].

The Pseudomonas putida rpl L gene coding for ribosomal protein L7/L12 was cloned and sequenced. Although Asp55 residue in L7/L12 was previously shown to be conservative in ten different organisms, the Pseudomonas putida L7/L12 proved to contain Asn55, thus showing that Asp55 is not invariant.

[Genes coding for RNA-polymerase in bacteria. II. Conservative sites in the central region of the beta-subunit of Pseudomonas putida RNA-polymerase].

SalI--L fragment of the P. putida rpoBC operon has been sequenced and conservative regions of the central part of the RNA-polymerase beta-subunit have been determined. Amino and acid residues interacting with Zn2+ are postulated.

Family of human Na+, K+-ATPase genes. Structure of the gene for the catalytic subunit (alpha III-form) and its relationship with structural features of the protein.

The primary structure of a gene of the Na+, K+-ATPase multigenic family in the human genome has been determined. The gene corresponds to a hypothetical alpha III-form of the enzyme catalytic subunit. The gene comprises over 25,000 bp, and its protein coding region includes 23 exons and 22 introns.

[Genes encoding bacterial RNA-polymerase. I. Cloning of rpoBC-operon of Pseudomonas putida and its physical map].

The P. putida rpoBC operon, coding for beta and beta' subunits of RNA polymerase, was cloned and its physical map constructed.

[Bank of sequences specific for human genome. I. The structure of one of the repeats in the Alu family].

A bank enriched in sequences specific for the human genome was obtained. In course of the analysis, a clone containing an Alu family repeat was identified and its primary structure determined.

The family of human Na+,K+-ATPase genes. No less than five genes and/or pseudogenes related to the alpha-subunit.

Five different nucleotide sequences have been found in the human genome homologous to the gene of the alpha-subunit of Na+,K+-ATPase. A comparative analysis of the primary structure of these genes in the region 749-1328 (in coordinates of cDNA from the pig alpha-subunit) is presented.

An improved technique for the efficient construction of gene libraries by partial filling-in of cohesive ends.

For the preparation of gene libraries, DNA from lambda EMBL3 phage was digested with SalI and EcoRI, and the cohesive ends partially filled-in by addition of dTTP, dCTP and Klenow fragment of DNA polymerase I (PolIk). Genomic DNA was cleaved partially with Sau3A and subsequently incubated with dATP, dGTP and PolIk. The phage and genomic DNAs were then mixed and ligated.

[Construction of a gene library using partial filling of DNA sticky ends].

To prepare gene libraries, the incomplete filling of protruding ends has been used. DNAs from phages EMBL 3 and EMBL 3a were sequentially digested with SalI and EcoRI, followed by addition of dTTP, dCTP, and DNA polymerase I (Klenow's fragment). Separately, a genomic DNA was partially cleaved with Sau3AI, followed by addition of dATP, dGTP, and Klenow's fragment.