Endosomal disentanglement of a transducible artificial transcription factor targeting endothelin receptor A.

Cell-penetrating peptides (CPPs) hold great promise for intracellular delivery of therapeutic proteins. However, endosomal entrapment of transduced cargo is a major bottleneck hampering their successful application. While developing a transducible zinc finger protein-based artificial transcription factor targeting the expression of endothelin receptor A, we identified interaction between the CPP and the endosomal membrane or endosomal entanglement as a main culprit for endosomal entrapment.

Scanning Thermal Microscopy and Ballistic Phonon Transport in Lateral Spin Valves.

Using scanning thermal microscopy, we have mapped the spatial distribution of temperatures in an operating nanoscale device formed from a magnetic injector, an Ag connecting wire, and a magnetic detector. An analytical model explained the thermal diffusion over the measured temperature range (2-300 K) and injector-detector separation (400-3000 nm). The characteristic diffusion lengths of the Peltier and Joule heat differ remarkably below 60 K, a fact that can be explained by the onset of ballistic phonon heat transfer in the substrate.

Beyond the compact magnetic domain wall.

Domain wall dynamics in wide submicrometer wires is investigated to understand the fundamental mechanisms that limit wall mobility, both experimentally by magneto-optical Kerr effect and by micromagnetic simulations. It is found that the dynamic domain wall structure departs significantly from the current description of a compact entity when evolving along the wire. The wall is composed of several substructures, each one propagating and evolving in a different dynamic regime with very different velocities.

Magnetologic devices fabricated by nanostencil lithography.

We present magnetic quantum cellular automata (MQCA), fabricated by means of nanostencil lithography, i.e., using a resistless shadow masking technique in ultra-high vacuum.

Safety and immunotoxicity assessment of immunomodulatory monoclonal antibodies.

Most therapeutic monoclonal antibodies (mAbs) licensed for human use or in clinical development are indicated for treatment of patients with cancer and inflammatory/autoimmune disease and as such, are designed to directly interact with the immune system. A major hurdle for the development and early clinical investigation of many of these immunomodulatory mAbs is their inherent risk for adverse immune-mediated drug reactions in humans such as infusion reactions, cytokine storms, immunosuppression and autoimmunity. A thorough understanding of the immunopharmacology of a mAb in humans and animals is required to both anticipate the clinical risk of adverse immunotoxicological events and to select a safe starting dose for first-in-human (FIH) clinical studies.

Electric field induced magnetic anisotropy in a ferromagnet.

We report the first observation of a transient all electric field induced magnetic anisotropy in a thin film metallic ferromagnet. We generate the anisotropy with a strong (approximately 10(9) V/m) and short (70 fs) E-->-field pulse. This field is large enough to distort the valence charge distribution in the metal, yet its duration is too brief to change the atomic positions.

Dependence of domain-wall depinning threshold current on pinning profile.

We have investigated the threshold current density required for depinning a domain wall from constrictions in NiFe nanowires, which give rise to pinning potentials of fixed amplitude but variable profile. We observed it to vary linearly with the angle of the triangular constriction. These results are reproduced using micromagnetic simulations including the adiabatic and nonadiabatic spin-torque terms.

Control of domain wall polarity by current pulses.

Direct observation of current-induced propagation of purely transverse magnetic domain walls with spin-polarized scanning electron microscopy is reported in Fe30Ni70 nanowires. After propagation, the domain walls keep their transverse nature but switch polarity in some cases. For uniform Ni70Fe30 wires, the effect is random and illustrates domain-wall propagation above the Walker threshold.

Atypical GPI-anchored T-cadherin stimulates angiogenesis in vitro and in vivo.

Objective: T-cadherin (T-cad) is an atypical GPI-anchored member of the cadherin superfamily. In vascular tissue, T-cad expression is increased during atherosclerosis, restenosis, and tumor neovascularization. In vitro, overexpression and/or homophilic ligation of T-cad on endothelial cells (ECs) facilitates migration, proliferation, and survival.

T-cadherin protects endothelial cells from oxidative stress-induced apoptosis.

In vascular tissue, T-cadherin (T-cad) is up-regulated in vivo under disease conditions associated with oxidative stress and concomitant cell migration, proliferation and apoptosis/survival. Using cultures of human umbilical vein endothelial cells (HUVEC), we examined whether there is a functional relationship between oxidative stress, T-cad expression, and cell survival status. Culture of HUVEC under conditions of oxidative stress (e.

Direct observation of domain-wall configurations transformed by spin currents.

Direct observations of current-induced domain-wall propagation by spin-polarized scanning electron microscopy are reported. Current pulses move head-to-head as well as tail-to-tail walls in submicrometer Fe20Ni80 wires in the direction of the electron flow, and a decay of the wall velocity with the number of injected current pulses is observed. High-resolution images of the domain walls reveal that the wall spin structure is transformed from a vortex to a transverse configuration with subsequent pulse injections.

RhoA and Rac mediate endothelial cell polarization and detachment induced by T-cadherin.

T-cadherin (T-cad) is an atypical GPI-anchored member of the cadherin superfamily. Ligation of T-cad receptors on endothelial cells prevents cell spreading, promotes elongation and polarization, decreases adhesion to the matrix, and facilitates migration. This study investigates involvement of Rho GTPases in T-cad signaling.

T-cadherin upregulation correlates with cell-cycle progression and promotes proliferation of vascular cells.

Objective: In vascular tissue, T-cadherin (T-cad) levels correlate with the progression of atherosclerosis, restenosis and tumour neovascularization. This study investigates whether T-cad influences proliferation of vascular cells.

Methods And Results: Cultures of human umbilical vein endothelial cells (HUVEC) and rat and human aortic smooth muscle cells (rSMC, hSMC) were used.

Direct observation of charge order in an epitaxial NdNiO3 film.

The first direct observation of charge order of Ni(3+delta(')) and Ni(3-delta) by resonant x-ray scattering experiments in an epitaxial film of NdNiO3 is reported. A quantitative value of delta+delta(') = (0.45 +/- 0.

Minimum field strength in precessional magnetization reversal.

Ultrafast magnetic field pulses as short as 2 picoseconds are able to reverse the magnetization in thin, in-plane, magnetized cobalt films. The field pulses are applied in the plane of the film, and their direction encompasses all angles with the magnetization. At a right angle to the magnetization, maximum torque is exerted on the spins.