Publications by authors named "Allen Chilun Luo"
Article Synopsis
- Mural cells play a crucial role in maintaining blood vessel integrity and function, and this study focuses on enhancing their generation from stem cells.
- Researchers employed the transcription factor NKX3.1 to effectively differentiate human induced pluripotent stem cells into mural progenitor cells, which is a new method compared to traditional growth factor approaches.
- The resulting iMPCs show important properties of mural cells, like contractility and matrix deposition, and may have significant potential in future vascular and regenerative medicine applications.
Article Synopsis
- The study introduces an efficient method for turning human induced pluripotent stem cells (iPSCs) into functional endothelial cells (iECs) using a doxycycline-inducible system to express the ETV2 transcription factor, achieving over 90% efficiency in just four days.
- This new method is designed to overcome challenges found in traditional transfection techniques, making it simpler and more consistent across different stem cell lines while allowing the iECs to maintain their ability to form blood vessels both in lab settings and in living organisms.
- The generated iECs show strong similarities to primary endothelial cells in terms of gene expression and protein profiles, validating their functionality and potential for use in research and
Article Synopsis
- Ischaemic diseases like critical limb ischaemia and heart attacks affect millions and endothelial cell (EC) transplants show promise in treatment but require support from other cells, complicating their use.
- This study found that mesenchymal stromal cells (MSCs) help ECs by transferring mitochondria via tunneling nanotubes, which is crucial for EC survival and function.
- Researchers developed a method to transplant mitochondria directly into ECs, enhancing their energy levels and promoting vessel formation without MSCs, while discovering that this process involves autophagy and the PINK1-Parkin pathway.
Human leukocyte antigen (HLA) genes play pivotal roles in numerous immunological applications. Given the immense number of polymorphisms, achieving accurate high-throughput HLA typing remains challenging. This study aimed to harness the human pan-genome reference consortium (HPRC) resources as a potential benchmark for HLA reference materials.
View Article and Find Full Text PDFCell transplantation success for myocardial infarction (MI) treatment is often hindered by low engraftment due to washout effects during myocardial contraction. A clinically viable biomaterial that enhances cell retention can optimize intramyocardial cell delivery. In this study, a therapeutic cell delivery method is developed for MI treatment utilizing a photocrosslinkable gelatin methacryloyl (GelMA) hydrogel.
View Article and Find Full Text PDFAdaptive immune receptor repertoire (AIRR) is encoded by T cell receptor (TR) and immunoglobulin (IG) genes. Profiling these germline genes encoding AIRR (abbreviated as gAIRR) is important in understanding adaptive immune responses but is challenging due to the high genetic complexity. Our gAIRR Suite comprises three modules.
View Article and Find Full Text PDFHereditary optic neuropathy (HON) is a group of genetically heterogeneous diseases that cause optic nerve atrophy and lead to substantial visual impairment. HON may present with optic nerve atrophy only or in association with various systemic abnormalities. Although a genetic survey is indispensable for diagnosing HON, conventional sequencing techniques could render its diagnosis challenging.
View Article and Find Full Text PDFInherited retinal degenerations (IRDs) are a group of phenotypically and genotypically heterogeneous disorders with substantial socioeconomic impact. In this cohort study, we tried to address the genetic characteristics and epidemiology of IRDs in Taiwan. Totally, 312 families with IRDs were identified and recruited and genetic testing was performed via probe capture-based NGS targeting 212 IRD-related genes.
View Article and Find Full Text PDF