Efficiency and Target Derepression of Anti-miR-92a: Results of a First in Human Study.

MicroRNA (miRNA) inhibition is a promising therapeutic strategy in several disease indications. MRG-110 is a locked nucleic acid-based antisense oligonucleotide that targets miR-92a-3p and experimentally was shown to have documented therapeutic effects on cardiovascular disease and wound healing. To gain first insights into the activity of anti-miR-92a in humans, we investigated miR-92a-3p expression in several blood compartments and assessed the effect of MRG-110 on target derepression.

Lysine (protein) requirements of lactating sows.

Five experiments were conducted to evaluate the lysine (Lys) requirements of lactating sows. All diets were formulated to be isocaloric 3.46 Mcal ME/kg and met or exceeded National Research Council recommendations.

Effects of different antibiotic feeding programs on morbidity and mortality and growth performance of nursery pigs housed in a wean-to-finish facility.

The objective of this study was to evaluate the effects of two antibiotic feeding programs in comparison to nonmedicated controls on the incidence of morbidity and mortality and growth performance of nursery pigs in a commercial setting. The study used 2,250 crossbred pigs in a randomized complete block design (blocking factor = start date). There were two dietary phases with three treatments in each phase: 1) nonmedicated controls vs.

The use of feed-grade amino acids in lactating sow diets.

Background: The use of feed grade amino acids can reduce the cost of lactation feed. With changing genetics, increasing feed costs, and higher number of pigs weaned with heavier wean weights further evaluation of higher inclusion levels of feed-grade amino acid in lactation diets than previously published is warranted. Two experiments (Exp.

Evaluation of the optimal standardized ileal digestible tryptophan:lysine ratio in lactating sow diets.

Three hundred and fifteen primiparous and multiparous sows were evaluated in a study to determine the effect of standardized ileal digestible (SID) Trp:Lys ratio in lactating sow diets. Camborough sows (PIC USA, Hendersonville, TN) ranging from first parity to eighth parity were blocked by parity and randomly allotted to 1 of 4 experimental diets containing different levels of added L-Trp (0.006, 0.

Effect of narasin (Skycis) or zinc bacitracin (Albac) inclusion on the growth performance and carcass characteristics of finishing pigs sent for slaughter using a 3-phase marketing strategy.

The objective of this study was to evaluate the effect of dietary inclusion of narasin or zinc bacitracin on the growth performance and carcass characteristics of finishing pigs sent for slaughter using a 3-phase marketing strategy. The study used 2,219 crossbred pigs in a randomized complete block design (blocking factor = start date) with 3 dietary treatments: 1) Control (no feed additive), 2) 15 mg/kg narasin (Skycis, Elanco Animal Health, Greenfield, IN), and 3) 28 mg/kg zinc bacitracin (Albac, Zoetis, Parsippany, NJ). Pigs were housed in single-sex pens of 25 pigs in a commercial wean-to-finish facility and there were 30 pen-replicates of each dietary treatment.

Effects of ractopamine hydrochloride on the growth performance and carcass characteristics of heavy-weight finishing pigs sent for slaughter using a 3-phase marketing strategy.

A total of 2,158 crossbred pigs was used to evaluate the effects of feeding 7.4 mg/kg ractopamine hydrochloride (RAC) on the growth performance and carcass characteristics of heavy-weight finishing pigs sent to slaughter using a 3-phase marketing strategy. The study was performed from 121.

The feeding of dried distillers' grains with solubles to lactating sows.

Three experiments were conducted to evaluate the feeding of dried distillers' grains with solubles (DDGS) in sow lactation diets. In Exp. 1, 168 multiparous sows (PIC, Camborough 22) were fed a 10% DDGS diet throughout gestation.

Ham and belly processing characteristics of immunological castrated barrows (Improvest) fed ractopamine hydrochloride (Paylean).

Effects of sex class (physically castrated, PC or immunologically castrated, IC) and diet (0 or 5mg/kg ractopamine hydrochloride, RAC) on characteristics of ham and bellies were determined from pigs slaughtered in three groups with similar ending live weights. One carcass per pen per marketing group (n=8) was selected to evaluate further processing characteristics. Data were analyzed as a 2×2 factorial design with a split plot in time and fixed effects of sex, diet, marketing group, and their interactions.

Effects of narasin (Skycis) on live performance and carcass traits of finishing pigs sold in a three-phase marketing system.

The objective was to evaluate the effect of feeding narasin (Skycis; Elanco Animal Health, Greenfield, IN) on growth performance and carcass characteristics of finishing pigs sold in a 3-phase marketing system. Pigs ( = 1,232) were housed in 56 single-sex pens (22 pigs/pen) divided into 2 even blocks based on initiation of treatment. Each treatment × sex combination was replicated 14 times.

Impact of Ivabradine on Central Aortic Blood Pressure and Myocardial Perfusion in Patients With Stable Coronary Artery Disease.

Unlabelled: Treatment of hypertensive patients with β-blockers reduces heart rate and decreases central blood pressure less than other antihypertensive drugs, implying that reducing heart rate without altering brachial blood pressure could increase central blood pressure, explaining the increased cardiovascular risk reported with β-blocker. We describe a randomized, double-blind study to explore whether heart rate reduction with the If inhibitor ivabradine had an impact on central blood pressure. We included 12 normotensive patients with stable coronary artery disease, heart rate ≥70 bpm (sinus rhythm), and stable background β-blocker therapy.

Effects of a chelated copper as growth promoter on performance and carcass traits in pigs.

Three studies were conducted to investigate whether a chelated Cu can replace CuSO4 as a growth promoter in pigs. In Exp. 1, a total of 240 piglets (Large White×Landrace, 7.

Effects of feeding ractopamine hydrochloride (Paylean) to physical and immunological castrates (Improvest) in a commercial setting on growth performance and carcass characteristics.

Growth performance and carcass characteristics of physically castrated (PC) and immunologically castrated (IC) pigs fed ractopamine hydrochloride (RAC; 5 mg/kg) were evaluated in 64 pens of 22 pigs each. Male pigs were randomly assigned to castration method at birth. Pigs in the PC group were physically castrated at 5 d of age while IC pigs were administered Improvest at 11 and 18 wk of age.

Effects of feeding ractopamine hydrochloride (Paylean) to physical and immunological castrates (Improvest) in a commercial setting on carcass cutting yields and loin quality.

Effects of feeding ractopamine (RAC; 5 mg/kg) to physically castrated (PC) and immunologically castrated (IC) pigs on carcass characteristics, cutting yields, and loin quality were evaluated using 285 carcasses. Male pigs were randomly assigned to sex treatments (PC and IC) at birth and fed the same nursery diets before allotment into 32 pens with 22 pigs per pen in a grow-finish barn. Pigs in the PC group were physically castrated at approximately 5 d of age, and pigs in the IC group were administered Improvest at 11 and 18 wk of age.

Impact of ractopamine hydrochloride on growth, efficiency, and carcass traits of finishing pigs in a three-phase marketing strategy.

The objectives were to determine the effects of ractopamine hydrochloride (RAC) in a 3-phase marketing strategy. One thousand seven hundred forty pigs were used in 80 single-sex pens in 2 blocks. Each pen housed approximately 22 pigs.

Effects of dietary soybean oil on pig growth performance, retention of protein, lipids, and energy, and the net energy of corn in diets fed to growing or finishing pigs.

The objectives of this experiment were 1) to determine if dietary soybean oil (SBO) affects the NE of corn when fed to growing or finishing pigs, 2) to determine if possible effects of dietary SBO on the NE of corn differ between growing and finishing pigs, and 3) to determine effects of SBO on pig growth performance and retention of energy, protein, and lipids. Forty-eight growing (initial BW: 27.3 ± 2.

Effects of dietary soybean hulls and wheat middlings on body composition, nutrient and energy retention, and the net energy of diets and ingredients fed to growing and finishing pigs.

The objectives of this experiment were 1) to determine the effect of dietary soybean hulls (SBH) and wheat middlings (WM) on body composition, nutrient and energy retention, and the NE of diets and ingredients fed to growing or finishing pigs and 2) to determine if finishing pigs use the energy in SBH and WM more efficiently than growing pigs. Forty growing barrows (initial BW: 25.4 ± 0.

Evaluation of standardized ileal digestible lysine requirement of nursery pigs from seven to fourteen kilograms.

Four experiments were conducted to determine the standardized ileal digestible (SID) Lys requirement of pigs (Sus scrofa) from 7 to 14 kg. In Exp. 1, 294 pigs (6.

Effect of corn dried distillers grains with solubles, conjugated linoleic acid, and ractopamine (paylean) on growth performance and fat characteristics of late finishing pigs.

An experiment was performed to evaluate effects of dietary ractopamine, CLA, and corn distillers dried grains with solubles (DDGS) on growth and carcass and fat quality of finishing pigs. This study was conducted as a split-split plot arrangement in a generalized randomized block design. In total 1,102 crossbred barrows and gilts (initial BW = 100.

The effect of corn distiller's dried grains with solubles, ractopamine, and conjugated linoleic acid on the carcass performance, meat quality, and shelf-life characteristics of fresh pork following three different storage methods.

The objective of this study was to evaluate dietary corn distiller's dried grains with solubles (DDGS), ractopamine hydrochloride (RAC), and conjugated linoleic acid (CLA) on growth performance, carcass and fat quality, and shelf-life of fresh pork from finishing pigs. Barrows (n=72) were fed one of eight treatments consisting of two diet sources (corn-soy and corn-soy+20% DDGS), two levels of RAC (0 and 7.4ppm), and two levels of CLA (0 and 0.

Fatty acid profiles and iodine value correlations between 4 carcass fat depots from pigs fed varied combinations of ractopamine and energy.

A total of 54 finishing barrows (initial BW = 99.8 ± 5.1 kg; PIC C22 × 337) reared in individual pens were allotted to 1 of 6 dietary treatments in a 2 × 3 factorial arrangement of treatments with 2 levels of ractopamine (0 and 7.

Impact of dietary energy level and ractopamine on growth performance, carcass characteristics, and meat quality of finishing pigs.

A total of 54 finishing barrows (initial BW = 99.8 ± 5.1 kg; PIC C22 × 337) reared in individual pens were allotted to 1 of 6 dietary treatments in a 2 × 3 factorial arrangement of treatments with 2 levels of ractopamine (0 and 7.

Estimation of the standardized ileal digestible valine-to-lysine ratio in 13- to 32-kilogram pigs.

Three experiments were conducted to determine the optimum standardized ileal digestible Val-to-Lys (SID Val:Lys) ratio for 13- to 32-kg pigs. In Exp. 1, 162 pigs weaned at 17 d of age (8 pens/treatment) were used, and a Val-deficient basal diet containing 0.

Net energy of soybean oil and choice white grease in diets fed to growing and finishing pigs.

The objectives of this experiment were 1) to determine the NE of soybean oil (SBO) and choice white grease (CWG) fed to growing and finishing pigs, 2) to evaluate the effects of inclusion rate of SBO on the NE by growing and finishing pigs, and 3) to determine if there is a difference in the NE of SBO and CWG between growing and finishing pigs. Forty-eight growing (initial BW: 22.13 ± 1.

Fresh meat and further processing characteristics of ham muscles from finishing pigs fed ractopamine hydrochloride.

Ractopamine hydrochloride (RAC) has consistently led to an advantage in carcass cutting yields of finishing pigs and remains a common feed additive in US finishing pig diets. Less is known about the effect of RAC on further processing characteristics. Some researchers have reported advantages in ultimate pH of the LM in pigs fed RAC.