Publications by authors named "Allan J Lepine"
To investigate temporal dynamic changes in the synthesis of chondroitin 6-sulfate (CS6) and chondroitin 4-sulfate (CS4) in vitro, normal articular cartilage of femoral heads was harvested from three dogs. Chondrocytes were isolated and cultured in alginate microspheres for 21 days. On days 7, 14 and 21, DNA content was quantified by fluorometric assay using Hoechst 33258.
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- Long-term athletic training in dogs, specifically English pointers and Labrador retrievers, leads to changes in muscle protein turnover, particularly concerning the ubiquitin-proteasome (UP) pathway, which is connected to muscle breakdown and repair.
- A study examined 44 hunting dogs by collecting muscle biopsies before and during peak activity seasons to analyze the activity levels of the UP pathway and other muscle-related factors.
- Results showed an increase in certain components of the UP pathway and muscle glycogen levels during peak activity, suggesting that these biological changes contribute to muscle adaptation in response to rigorous athletic training.
Objective: To study the musculoskeletal development of Great Dane puppies fed various dietary concentrations of calcium (Ca) and phosphorus (P) in fixed ratio by use of dual energy x-ray absorptiometry (DEXA), determination of serum insulin-like growth factor 1 and parathyroid hormone concentrations, radiography, and blood chemistry analysis results.
Animals: 32 purebred Great Dane puppies from 4 litters.
Procedure: At weaning, puppies were assigned randomly to 1 of 3 diets.