Breaking the Restriction Barriers and Applying CRISPRi as a Gene Silencing Tool in .

is a thermophilic bacterium capable of producing succinate from lignocellulosic-derived sugars and has the potential to be exploited as a platform organism. However, exploitation of has been limited partly due to the genetic inaccessibility and lack of genome engineering tools. In this study, we established the genetic accessibility for DSM 5809.