Standardized high-sensitivity flow cytometry testing for paroxysmal nocturnal hemoglobinuria in children with acquired bone marrow failure disorders: A single center US study.

Background: Paroxysmal nocturnal hemoglobinuria (PNH) is a rare, acquired hematopoietic stem cell disorder that has not been well-documented in children, particularly those with acquired bone marrow failure disorders (ABMFD)-acquired aplastic anemia (AAA) and myelodysplastic syndrome (MDS). Therefore, we sought to determine the prevalence of PNH populations in children with ABMFD.

Methods: PNH testing was performed in children with an ABMFD diagnosis using high sensitivity (≥0.

Extracellular matrix scaffold for cardiac repair.

Background: Heart failure remains a significant problem. Tissue-engineered cardiac patches offer potential to treat severe heart failure. We studied an extracellular matrix scaffold for repairing the infarcted left ventricle.

Implications of p53 in growth arrest and apoptosis on combined treatment of human Mammary epithelial cells with topotecan and UCN-01.

We previously reported (Cancer Chemother Pharmacol 45: 252-258, 2000) that UCN-01 (7-hydroxystaurosporine), a protein kinase inhibitor, which is under clinical trials as an anti-cancer agent in the USA and Japan, enhanced camptothecin-induced cytotoxicity in breast cancer cells that lack p53 function. This enhancement was mediated by the abrogation of G2 arrest of tumor cells. Subsequent studies from our laboratory also revealed that the combined use of both UCN-01 and camptothecin induced DNA double strand breaks in p53 mutant tumor cells but not in normal or p53 negative epithelial cells.

Resveratrol is a potent inducer of apoptosis in human melanoma cells.

Resveratrol is a plant polyphenol found in grapes and red wine. It has been found to have beneficial effects on the cardiovascular system. Resveratrol also inhibits the growth of various tumor cell lines in vitro and inhibits carcinogenesis in vivo.