Assessment of micronuclei frequency in individuals with a habit of tobacco by means of exfoliated oral buccal cells.

Aims And Objectives: To study the genotoxic effects of tobacco on the exfoliated buccal epithelial cells in patients with oral precancerous lesions (OPLs) and Patients with tobacco habit but without oral precancerous lesion(habit controls) by using micronucleus assay as well as the quantification and detection of the biomarkers in these premalignant lesions which will be helpful in finding those patients who are at higher risk for malignant transformation.

Materials And Methods: Forty samples were collected from the right and left side of buccal epithelial cells obtained from 20 individuals, i.e.