Publications by authors named "Alison Vinitsky"
Article Synopsis
- Bi-functional N-Hydroxysuccinimide (NHS) linkers are crucial in connecting immunogens with carrier proteins to enhance immune responses, particularly in HIV-1 vaccine development.
- The study investigated the degradation kinetics of these linkers using reversed-phase liquid chromatography (RPLC-UV) to ensure effective conjugation before the linkers become inactive.
- Three types of cross-linkers were examined for their degradation pathways, with specific kinetics reported for the Sulfosuccinimidyl (4-iodoacetyl) aminobenzoate (Sulfo-SIAB) linker.
Article Synopsis
- Conjugate-vaccine immunogens consist of a carrier protein, an antigen, and a crosslinker that connects the two while maintaining immune responses; this study focuses on the effects of different crosslinkers between an HIV-1 antigen (FP8) and the carrier protein (rTTHC).
- The research evaluated various physical properties and immunogenic responses of seven different FP8-rTTHC conjugates with varying linker lengths and types, finding that most conjugates were well recognized by specific HIV-fusion-peptide antibodies, except those with the shortest and longest linkers, which showed reduced recognition.
- Immunization tests in mice showed that conjugates with shorter (SIA) and
One of the HIV-1 vaccine design efforts has focused on developing a recombinant HIV-1 trimeric envelope glycoprotein (Env) as an immunogen to induce broadly neutralizing antibodies. A native-like immunogen, the BG505.DS.
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