Pediatric glioma histone H3.3 K27M/G34R mutations drive abnormalities in PML nuclear bodies.

Background: Point mutations in histone variant H3.3 (H3.3K27M, H3.

Active maintenance of CD8 T cell naivety through regulation of global genome architecture.

The differentiation of naive CD8 T lymphocytes into cytotoxic effector and memory CTL results in large-scale changes in transcriptional and phenotypic profiles. Little is known about how large-scale changes in genome organization underpin these transcriptional programs. We use Hi-C to map changes in the spatial organization of long-range genome contacts within naive, effector, and memory virus-specific CD8 T cells.

Active maintenance of CD8 T cell naïvety through regulation of global genome architecture.

The differentiation of naïve CD8 cytotoxic T lymphocytes (CTLs) into effector and memory states results in large scale changes in transcriptional and phenotypic profiles. Little is known about how large-scale changes in genome organisation reflect or underpin these transcriptional programs. We utilised Hi-C to map changes in the spatial organisation of long-range genome contacts within naïve, effector and memory virus-specific CD8 T cells.

3D Single Molecule Super-Resolution Microscopy of Whole Nuclear Lamina.

Single molecule (SM) super-resolution microscopies bypass the diffraction limit of conventional optical techniques and provide excellent spatial resolutions in the tens of nanometers without overly complex microscope hardware. SM imaging using optical astigmatism is an efficient strategy for visualizing subcellular features in 3D with a -range of up to ∼1 µm per acquisition. This approach however, places high demands on fluorophore brightness and photoswitching resilience meaning that imaging entire cell volumes in 3D using SM super-resolution remains challenging.

CD4 T help promotes influenza virus-specific CD8 T cell memory by limiting metabolic dysfunction.

There is continued interest in developing novel vaccine strategies that induce establish optimal CD8 cytotoxic T lymphocyte (CTL) memory for pathogens like the influenza A viruses (IAVs), where the recall of IAV-specific T cell immunity is able to protect against serologically distinct IAV infection. While it is well established that CD4 T cell help is required for optimal CTL responses and the establishment of memory, when and how CD4 T cell help contributes to determining the ideal memory phenotype remains unclear. We assessed the quality of IAV-specific CD8 T cell memory established in the presence or absence of a concurrent CD4 T cell response.