Quantitation of Phenolic Benzotriazole Class Compounds in Plasma by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS).

Phenolic benzotriazoles are used as UV stabilizers in consumer products and have been detected in the environment suggesting potential human exposure. Phenolic benzotriazoles were nominated to the Division of National Toxicology Program for testing based on their potential widespread human exposure and lack of adequate toxicity data. Nine chemicals were selected for toxicological evaluation, representing unsubstituted (2-(2H-benzotriazole-2-yl)phenol, (P-BZT)), monosubstituted (drometrizole; 2-(2H-benzotriazol-2-yl)-4-tert-butylphenol (tBu-BZT); octrizole), disubstituted (2-(2H-benzotriazol-2-yl)-4,6-bis(1-methyl-1-phenylethyl)phenol (diMeEtPh-BZT), 2-(2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylpropyl)phenol (ditPe-BZT); 3-(2H-benzotriazol-2-yl)-5-(1,1-dimethylethyl)-4-hydroxybenzenepropanoic acid, octylester (tBuPrOcEst-BZT) and halogenated trisubstituted (bumetrizole; 2-(5-chloro-2H-benzotriazol-2-yl)-4,6-bis(1,1-dimethylethyl)phenol (ditBuCl-BZT)) compounds.

Development and Validation of an Analytical Method to Quantitate Hydroxycitric Acid, the Key Constituent in Extract, in Rodent Plasma and Fetus.

extract (GCE) is a popular botanical supplement used in weight loss products. Hydroxycitric acid (HCA) is the principal component in GCE. Due to lack of adequate toxicity data to assess the safe use of GCE, the National Toxicology Program is testing GCE in Hsd:Sprague Dawley® SD® rats following perinatal exposure and in adult B6C3F1/N mice.