Multiple biomarkers response of Astyanax lacustris (Teleostei: Characidae) exposed to manganese and temperature increase.

The present study aimed to evaluate the toxicity of Mn (6.65 mg/L) at different exposure times (96 h, 7, 14, and 21 days) and evaluate its possible toxic effects on the fish Astyanax lacustris through multi-biomarkers and the maximum critical temperature (CT Max). The results show an increase in the Mn accumulation (liver and gills) with increasing exposure time.

Gene expression changes in Epinephelus marginatus (Teleostei, Serranidae) liver reveals candidate molecular biomarker of iron ore contamination.

Wastes from iron ore mining activities are potentially damaging to adjacent aquatic ecosystems. We aimed to determine biomarkers of environmental exposure to this xenobiotic in the dusky grouper Epinephelus marginatus by differential gene expression analysis. For this, fish were exposed to iron ore (15.

Gene Expression Changes in Epinephelus marginatus (Teleostei, Serranidae) Liver as Molecular Biomarker of Iron Ore Contamination.

The aim of this study was to determine selected molecular biomarkers of iron ore contamination in Epinephelus marginatus. Molecular biomarkers were tested by checking the relative expression of genes involved in oxidative stress, trace element regulation, and cellular damage, by RT-qPCR. Iron ore exposure caused the downregulation of ferroportin (FP) gene expression and a significant upregulation in superoxide dismutase (SOD) and cytochrome P450 1A (CYP1A) genes.

Why does your pain never get better? Stigma and coping mechanism in people with sickle cell disease.

Objectives: to analyze the characteristics of stigma in the interactions of people with pain and sickle cell disease and the coping strategies adopted.

Methods: qualitative study, conducted in Bahia's reference units between January and July 2018. In-depth interviews were applied to 25 adults, followed by content analysis and interpretation in light of the Sociological Theory of Stigma.

Construction and validation of a family guidance manual on complications of intravenous therapy in children.

Objectives: to build and validate the content of an educational manual for the inclusion of family members of hospitalized children in the prevention and early identification of complications associated with IVT through the peripheral route.

Methods: it was a methodological study of construction and validation of the content of an instructional material. The validation process took place using the Delphi Technique, adopting a content validation index equal to or greater than 0.

Systematic monitoring of glanders-infected horses by complement fixation test, bacterial isolation, and PCR.

Glanders is an equine zoonosis caused by that is responsible for considerable economic loss. Complement fixation testing (CFT) using warm or cold incubation are recommended by the OIE, but many routinely used detection tests may present misleading results. To increase accuracy of glanders diagnosis and establish an appropriate protocol in collaboration with the National Equine Health Program, seven horses positive for glanders kept in isolation in Brazil were examined fortnightly by CFT, microbiological screening, and molecular testing.

Geophagus brasiliensis (Teleostei: Cichlidae) as an indicator of toxicity of ornamental stone processing wastes.

Massive exploitation of geological resources may lead to environmental issues due to the inadequate disposal of the processing wastes, which are potentially hazard to terrestrial and aquatic environments. To evaluate the toxic effects ornamental stones processing wastes (OSPW), Geophagus brasiliensis fish were contaminated with different concentrations of OSPW (250, 500, 750 and 1000 mg/L). The contaminated aquarium water showed increased total hardness and Ca, Na, K, Mg and Mn content, which lead to bioconcentration of Na, K and Mg in G.

Toxic effects of ornamental stone processing waste effluents on Geophagus brasiliensis (Teleostei: Cichlidae).

The ornamental stone industry generates considerable amounts of waste (OSPW), which may eventually reach natural environments and impact the local ecosystem. The aim of this study was to compare the toxic effects of two OSPW effluents in Geophagus brasiliensis: i) leachate effluent from a lagoon in an OSPW landfill (LE) and ii) decanted effluent from an ornamental stone processing industry (DE). G.