Identification of Escherichia coli isolated from flies (Insecta: Diptera) that inhabit the environment of dairy farms harboring extraintestinal virulence markers.

Aims: We investigate extraintestinal pathogenic genes (ExPEC) related to virulence of Escherichia coli in flies from the dairy environment.

Methods And Results: We collected 217 flies from nine dairy farms, which were submitted to microbiological culture. Fifty-one E.

Flies (Insecta, Diptera) collected in the environment of dairy farms as carriers of Rotavirus A and betacoronavirus.

Aims: We aimed to investigate the prevalence of rotavirus and coronavirus in dipterans that commonly inhabit the environment of dairy farms.

Methods And Results: We collected 217 insect specimens from nine dairy farms, which were examined through hemi-nested RT-PCR followed by Sanger sequencing in search of VP1 and N genes for rotavirus and bovine coronavirus-BCoV, respectively. With a predominance of Muscidae (152/217 = 70%) 11 families of Diptera were identified.

Interactive Key for Third Instar Larvae of Neotropical Blow Flies (Insecta, Diptera, Calliphoridae): the Contribution of Computational Tools to Assist in Species Identification.

Calliphoridae (Insecta, Diptera), popularly known as blow flies or carrion flies, as most are necrophagous comprises one of the most relevant families of insects on forensics. Currently, more than 1500 blow fly species are known, the majority of which can be found in the Old World. In the Neotropics, it is estimated that there are approximately 20 species of forensic importance.

How much epigenetics and quantitative trait loci (QTL) mapping tell us about parasitism maintenance and resistance/susceptibility to hosts.

Interactions between the environment, parasites, vectors, and/or intermediate hosts are complex and involve several factors that define the success or failure of an infection. Among these interactions that can affect infections by a parasite, it is possible to highlight the genetic and epigenetic mechanisms in hosts and parasites. The interaction between genetics, epigenetics, infection, and the host's internal and external environment is decisive and dictates the outcome of a parasitic infection and the resistance, susceptibility, and transmission of this parasite.

First description of the immature stages of Fannia sabroskyi Seago, 1954 (Insecta, Diptera, Fanniidae).

Egg, larvae and the puparium of Fannia sabroskyi Seago, 1954 (Diptera, Fanniidae) are described and illustrated for the first time. The following structures have been documented: pseudocephalon, antennal complex, maxillary palpus, facial mask, anterior spiracle, cephaloskeleton structures, thoracic and abdominal processes, anal division, and posterior spiracle. Due to its necrophagous habit, this species may be of potential forensic importance for estimating the postmortem interval in criminal investigations.