Neonatal injections of methoxychlor decrease adult rat female reproductive behavior.

Methoxychlor (MXC), a commonly used pesticide, has been labeled as an endocrine disruptor. To evaluate the impact of neonatal exposure to MXC on female reproduction, female Sprague-Dawley rats were given subcutaneous injections on postnatal days 1, 3, and 5. The injections contained 1.

Loss of steroidogenic factor 1 alters cellular topography in the mouse ventromedial nucleus of the hypothalamus.

Knockout (KO) mice lacking the orphan nuclear receptor steroidogenic factor 1 (SF-1) exhibit marked structural abnormalities of the ventromedial nucleus of the hypothalamus (VMH). In this study, we sought to determine the molecular mechanisms underlying the VMH abnormalities. To trace SF-1-expressing neurons, we used a SF-1/enhanced green fluorescent protein (eGFP) transgene.

Differential colocalization of Islet-1 and estrogen receptor alpha in the murine preoptic area and hypothalamus during development.

Estrogen receptor (ER) expression and regulation is vital to the correct functioning of the neuroendocrine brain. Islet-1 (Isl-1) is a LIM homeodomain-containing transcription factor that has been implicated in neuronal differentiation, is located in the hypothalamus, and can alter ER function in vitro. We have determined that Isl-1 is localized in several regions of the hypothalamus, including the ER rich areas of the ventromedial nucleus (VMH), the preoptic area, and the anterior hypothalamus.

Gamma-aminobutyric acidB receptors and the development of the ventromedial nucleus of the hypothalamus.

Gamma-aminobutyric acid (GABA) is a highly abundant neurotransmitter in the brain and the ligand for GABA(A), GABA(B), and GABA(C) receptors. Unlike GABA(A) and GABA(C) receptors, which are chloride channels, GABA(B) receptors are G-protein linked and alter cell-signaling pathways. Electrophysiological studies have found GABA(B) receptors in cultured embryonic hypothalamus, but the distribution of these receptors remains unknown.