Alternative fertilizer-based growth media support high lipid contents without growth impairment in Scenedesmus obliquus BR003.

Nitrogen (N) sources have been target in microalgae cultivation studies, considering their nutritional impact on growth and high costs. Here, we have evaluated the growth of Scenedesmus obliquus BR003, applying alternative low-cost culture media containing ammonium and urea, or combinations of both N sources. The culture media were applied for indoor and outdoor cultivation, followed by growth analyses and metabolic characterization.

Terminal repeat retrotransposons as DNA markers in fungi.

In this study, we demonstrate that ClIRAP primers designed using the transposable element RetroCl1 sequence from Colletotrichum lindemuthianum can be used to generate an efficient IRAP (inter-retrotransposon amplified polymorphism) molecular marker to study intra- and inter-species diversity in fungi. It has been previously demonstrated that primers generated from this TRIM-like element can be used in the Colletotrichum species. We now prove that the RetroCl1 sequence can also be used to analyze diversity in different fungi.