[THROMBOTIC COMPLICATIONS AND LATENT HYPERCOAGULATION IN PATIENTS WITH BETA-THALASSEMIA].

The aim of the work is to investigate the clinical symptoms of thrombotic complications (TC) and the tendency of patients to increased thromb-formation in patients with beta-thalassemia (β-T). Female patients with β-T aged 18-40 years were studied: 130 beta-thalassemia major (β-TM), 95 patients beta-thalassemia intermediate (β-TI), 60 patients with beta-thalassemia minor (β-Tm). In patients with βT, the frequency of occurrence of TC was studied.

Myosin IIA and formin dependent mechanosensitivity of filopodia adhesion.

Filopodia, dynamic membrane protrusions driven by polymerization of an actin filament core, can adhere to the extracellular matrix and experience both external and cell-generated pulling forces. The role of such forces in filopodia adhesion is however insufficiently understood. Here, we study filopodia induced by overexpression of myosin X, typical for cancer cells.

[THE STATUS OF VITAMIN В UNDER DISEASES OF SMALL INTESTINE IN CHILDREN IN THE ZONE OF INCREASED SUN INSOLATION].

The study was carried out to trace clinical laboratory parallels between levels of vitamin D under diseases of small intestine in children. The examined sampling included 160 children aged from 6 months to 18 years with diseases of small intestine. The patients were separated to three groups: 60 children with celiac disease aged from 3-16 years; 60 children with chronic post-infectious enterocolitis aged from 2 to 12 years; 40 children with allergic enterocolitis aged from 6 months to 4 years.

[Urban air pollution with heavy metals and evaluating risk for public health].

The authors presented hygienic evaluation of ambient air in various districts of Baku city with heavy metals--lead, cadmium, chromium, nickel, copper and zinc. The metals contents of the air were assessed indirectly by their levels in soils of the areas under study. Findings are that soil levels of zinc, chromium and nickel exceeded those of the other metals by a degree.

[Results of YAG-laser vitreolysis in proliferative diabetic retinopathy complicated by vitreous hemorrhage].

Purpose: To determine the efficiency of YAG-laser vitreolysis in patients with diabetic vitreous hemorrhage.

Material And Methods: The study enrolled 56 patients (61 eyes) with proliferative diabetic retinopathy complicated by vitreous hemorrhage aged 36-69 years, including 31 women (34 eyes) and 25 men (27 eyes), who underwent YAG-laser vitreolysis. Assessment methods included visual acuity measurement, tonometry, ophthalmic biomicroscopy, glycated hemoglobin blood test and ultrasound exams (B-scan and quantitative echography).

[Ultrasonic diagnosis of functional and organic changes of colon in patients suffering from chronic constipation].

The aim of the study was to define the informative significance of USI in the diagnosis of functional and organic changes in patients suffering from chronic constipation (CC). 96 patients with CC were evaluated and treated. There were 67 women and 29 men (79) aged 33-55 years.

Involvement of the Rho-mDia1 pathway in the regulation of Golgi complex architecture and dynamics.

In mammalian cells, the Golgi apparatus is a ribbon-like, compact structure composed of multiple membrane stacks connected by tubular bridges. Microtubules are known to be important to Golgi integrity, but the role of the actin cytoskeleton in the maintenance of Golgi architecture remains unclear. Here we show that an increase in Rho activity, either by treatment of cells with lysophosphatidic acid or by expression of constitutively active mutants, resulted in pronounced fragmentation of the Golgi complex into ministacks.

Increased synapsin expression and neurite sprouting in lamprey brain after spinal cord injury.

Spinal cord injury induces structural plasticity throughout the mammalian nervous system, including distant locations in the brain. Several types of injury-induced plasticity have been identified, such as neurite sprouting, axon regeneration, and synaptic remodeling. However, the molecular mechanisms involved in injury-induced plasticity are unclear as is the extent to which injury-induced plasticity in brain is conserved across vertebrate lineages.

Regenerated synapses in lamprey spinal cord are sparse and small even after functional recovery from injury.

Despite the potential importance that synapse regeneration plays in restoring neuronal function after spinal cord injury (SCI), even the most basic questions about the morphology of regenerated synapses remain unanswered. Therefore, we set out to gain a better understanding of central synapse regeneration by examining the number, distribution, molecular composition, and ultrastructure of regenerated synapses under conditions in which behavioral recovery from SCI was robust. To do so, we used the giant reticulospinal (RS) neurons of lamprey spinal cord because they readily regenerate, are easily identifiable, and contain large synapses that serve as a classic model for vertebrate excitatory neurotransmission.

Diversity and evolution of coral fluorescent proteins.

GFP-like fluorescent proteins (FPs) are the key color determinants in reef-building corals (class Anthozoa, order Scleractinia) and are of considerable interest as potential genetically encoded fluorescent labels. Here we report 40 additional members of the GFP family from corals. There are three major paralogous lineages of coral FPs.

[Water electrolyte disorders in the postoperative clinical picture of basal-diencephalitic tumors].

The paper deals with the relationship of homeostatic and motivational disorders in the postoperative clinical picture in basal-diencephalic tumors. The material included 354 clinical observations of: hypophysial adenoma (n = 135); craniopharyngioma (n = 71); tumors of the third ventricle (n = 88); basal meningiomas (n = 60). A comparative analysis of the findings allowed the authors to support that there are typical variants of changes in the patients' status, which reflect the nature of ion-osmotic disorders.

Dynamics of contacts between lamellae of fibroblasts: essential role of the actin cytoskeleton.

We investigated actin cytoskeletal and adhesion molecule dynamics during collisions of leading lamellae of nontransformed and oncogene-transformed fibroblasts. By using real-time video microscopy, it was found that during lamellar collision there was considerable overlapping of leading lamellae followed by subsequent retraction. Overlapping of nontransformed fibroblasts was accompanied by formation of beta-catenin-positive contact structures organized into strands oriented parallel to the long axis of the cell that were associated with bundles of actin filaments.

Cell-cell contact changes the dynamics of lamellar activity in nontransformed epitheliocytes but not in their ras-transformed descendants.

We investigated the structural and functional alterations of active lamellae during initial cell-cell collision and establishment of cell-cell contacts in wounded cultures of nontransformed rat epitheliocytes (IAR-2 line) and their ras-transformed descendants (C4 line). Typically, the leading edges of nontransformed cells formed multiple transient contacts followed by establishment of small, stable contacts that would undergo lateral expansion. Formation and expansion of the contact area was accompanied by accumulation of the cell-cell adhesion molecules E-cadherin, beta-catenin, and plakoglobin.

[Mechanisms of the joint action of cortisol and adenosine on peripheral blood lymphocytes in bronchial asthma].

The effect of adenosine (10(-6) M) on the cyclic AMP production in lymphocytes of peripheral blood of patients with bronchial asthma and healthy donors was studied. Cells response to adenosine-induced stimulation was significantly decreased for asthmatic patients as compared to normal subjects. Cortisol (3 x 10(-6) M) was shown to be able to normalize suppressed function of cells of the patients.

[Role of monoaminoxidase in the intensification of peroxidation of lipids in mitochondria during experimental myocardial necrosis].

The relationship between lipid peroxidation and rat heart mitochondrial monoamine oxidase activity was studied in experimental myocardial necrosis induced by adrenaline injection. It has been established that both the intensity of peroxidation and the activity of monoamine oxidase in mitochondria from adrenaline-injured rat myocardium were essentially increased. The preliminary administration of antioxidants (vitamin E and ionol) was shown to decrease both the intensity of lipid peroxidation and the activity of monoamine oxidase.

[Effect of synthetic and natural inhibitors on the activity of tissue activator from the swine heart in vitro].

Activity of tissue activator, isolated from pig heart, was studied in vitro in presence of synthetic inhibitors epsilon-aminocapronic acid and amben as well as of natural inhibitor contrical. All the inhibitors studied inhibited the tissue activator as shown by analyses on fibrin plates and by means of quantitative estimation of thrombolytic activity in fibrinolytic preparations in vitro. Among the two synthetic inhibitors amben exhitited the highest effect on the tissue activator: its effect was 3-4-fold higher as compared with epsilon-aminocapronic acid.

[Effects of epsilon-aminocaproic acid, amben and kontrikal on fibrinolysis due to tissue-type plasminogen activator from the pig heart].

Simultaneous intravenous administration to rats of epsilon-aminocaproic acid and high doses of plasminogen tissue activator from the pig heart was shown to prevent fibrinolysis changes induced by the tissue activator. Amben completely suppressed the action of the tissue activator at the blood concentration 15 times less than that of epsilon-aminocaproic acid. A greater effect of amben on the blood activator level was noted.