Effect of addition of buserelin acetate to the extender on motility and viability of bovine spermatozoa.

The present study was aimed to determine the effect of GnRH analog (buserelin acetate) on the quality of bovine spermatozoa stored at 16° C for 24 h. Semen collected in the summer season from June to September from healthy Polish Holstein-Friesian bulls. Ejaculates were centrifuged, divided and diluted to the final concentration of 240 × 10 spermatozoa/mL using animal protein-free commercial BIOXcell extender (IMV Technologies, L'aigle, France) (Control) or with BIOXcell extender supplemented with buserelin acetate and stored 0, 8 and 24 h.