Publications by authors named "Alicja Batog"
Microsampling techniques enable the minimization of blood collection volume from animals and subsequent handling of the blood samples or their derived plasma or serum samples. This offers advantages over conventional large-volume sampling, such as eliminating the need for satellite animals and improving animal welfare aspects, and providing the opportunity for additional assessments in small animals where blood volume constraints limit endpoints. This study evaluated the feasibility of implementation of capillary microsampling (CMS) in a single-dose study in mice with the ultimate goal of enabling its use in toxicology studies.
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- The study aimed to measure the exposure of PRM-151, a recombinant form of human pentraxin-2, using a ligand-binding assay, but found issues with dose-dependent exposure in certain preclinical species and an inability to distinguish between infused PRM-151 and endogenous PTX-2 in primates.
- To address these challenges, the researchers implemented a more reliable LC-MS assay that avoids interference from PRM-151's various binding partners, allowing for the measurement of total drug levels without complicated capture reagents.
- The LC-MS results corroborated the original findings, revealing that the lack of dose-dependent exposure is due to the drug's complex binding and distribution in the body rather than a flaw in the assay technique.
Background: A defined approach to develop and validate an LC-MS/MS assay using dried blood spot (DBS) samples is of great interest to many scientists who are adopting this technology. We have evaluated three distinct sample preparation procedures of DBS samples for LC-MS/MS assay development.
Results: A new term 'elution efficiency' is introduced to evaluate the effectiveness of eluting compounds from the DBS cards into the liquid phase.