Sequencing confirms Anopheles stephensi distribution across southern Yemen.

The invasion of Anopheles stephensi in Africa warrants investigation of neighboring countries. In this study, genetic analysis was applied to determine the status of An. stephensi in southern Yemen.

The first record of the invasive mosquito species in Yemen.

The invasive mosquito is regarded as a public health threat due to its ability to transmit pathogens such as dengue and chikungunya viruses, its wide range of hosts, and its ecological plasticity. has already invaded parts of the Middle East region and further expansion is expected. Twenty-two sites in three districts of Yemen (Al Ghaydha, Hawf, and Sayhout) were screened for the presence of mosquitoes.

Effects of Seasonal Conditions on Abundance of Malaria Vector Anopheles stephensi Mosquitoes, Djibouti, 2018-2021.

We describe the influence of seasonal meteorologic variations and rainfall events on Anopheles stephensi mosquito populations during a 40-month surveillance study at a US military base in Djibouti. Focusing surveillance and risk mitigation for An. stephensi mosquitoes when climatic conditions are optimal presents an opportunity for malaria prevention and control in eastern Africa.

Crimean Congo Hemorrhagic Fever Virus and Alkhurma (Alkhumra) Virus in Ticks in Djibouti.

Crimean Congo hemorrhagic fever virus and Alkhumra virus, not previously reported in Djibouti, were detected among 141 (infection rate = 15.7 per 100, 95% CI: 13.4-18.

Evidence of Rickettsia and Orientia Infections Among Abattoir Workers in Djibouti.

Of 49 workers at a Djiboutian abattoir, eight (16%, 95% confidence interval [CI]: 9-29) were seropositive against spotted fever group rickettsiae (SFGR), two (4%, 95% CI: 1-14) against typhus group rickettsiae, and three (6%, 95% CI: 2-17) against orientiae. One worker (9%, 95% CI: 2-38) seroconverted against orientiae during the study period. This is the first evidence of orientiae exposure in the Horn of Africa.

Complete Genome Sequence of Chikungunya Virus Isolated from an Aedes aegypti Mosquito during an Outbreak in Yemen, 2011.

Chikungunya virus is recognized as a serious public health problem. The complete genome was sequenced for a chikungunya virus isolated from the mosquito Aedes aegypti during a 2011 outbreak in Al Hodayda, Yemen, which resulted in significant human fatalities. Phylogenetic analysis demonstrated that this Yemeni isolate is most closely related to Indian Ocean strains of the east/central/south African genotype.

Chikungunya outbreak in Al-Hudaydah, Yemen, 2011: epidemiological characterization and key lessons learned for early detection and control.

Little is known about the occurrence of chikungunya fever in the Eastern Mediterranean Region of the World Health Organization (WHO). In January 2011, the Ministry of Public Health and Population (MoPH&P) of Yemen reported to WHO an increasing number of "dengue-like" acute febrile illnesses of unknown origin from one of its coastal governorates. An epidemiological investigation was conducted in Al-Hudaydah governorate between 23 and 26 January 2011 by a joint team of WHO, the MoPH&P of Yemen and the U.

Infectivity of Metarhizium anisopliae (Hypocreales: Clavicipitaceae) to Phlebotomus papatasi (Diptera: Psychodidae) under laboratory conditions.

Susceptibility of Phlebotomus papatasi Scopoli (Diptera: Psychodidae) larvae to the entomopathogenic fungus Metarhizium anisopliae (Metschinkoff) Sorokin (Ma79) (Hypocreales: Clavicipitaceae) was evaluated at two different temperatures. The ability of the fungus to reinfect healthy sand flies was followed up for approximately 20 wk and the effect of in vivo repassage on the enhancement of its virulence was assessed. The fungus reduced the adult emergence at 26 +/- 1 degrees C when applied to larval diet.

Seasonal abundance, number of annual generations, and effect of an entomopathogenic fungus on Phlebotomus papatasi (Diptera: Psychodidae).

The monthly density of the sand fly, Phlebotomus Papatasi Scopoli (Diptera: Psychodidae), was monitored during 2009 at Burg El-Arab, a rural district located close to the Mediterranean coast of Egypt. The number of annual generations and the efficacy of microbial control by the entomopathogenic fungus, Metrahizium anisopliae (Metsch.) Sorok (Ma79), were determined in the laboratory under atmospheric conditions, simulating those of the animal shelters in the study area.

Sticky bottle traps: a simple and effective method for collecting adult phlebotomine sand flies from rodent burrows in a Leishmania-endemic region of Egypt.

We describe a simple, economic, and effective method for constructing sticky bottle traps that can be used to capture adult sand flies from rodent burrows. Although sand fly surveillance activities often employ light- or CO2-baited traps, sticky papers secured to a post or placed on the ground can also be used. However, in arid environments, sand and other debris often collect on the sticky surface, reducing trap effectiveness, capacity, and a means for rapid discrimination and enumeration of adult specimens.

Crimean-Congo hemorrhagic fever, Afghanistan, 2009.

In response to an outbreak of Crimean-Congo hemorrhagic fever in western Afghanistan, we measured immunoglobulin G seroprevalence among household members and their animals. Seroprevalence was 11.2% and 75.

Enzymatic changes and cytotoxic effects of Metarhizium anisopliae fungal extracts on the Egyptian cotton leaf worm Spodoptera littoralis (SL 96) cell line.

The extra-and intra-cellular extracts of two Metarhizium anisopliae isolates: Ma23 and MR3, were introduced to a cell line of Spodoptera littoralis. The cell line (Sl96) was established in the Entomovirology centre from S. littoralis ovaries.

Pathogenicity of two entomopathogenic hyphomycetes, Beauveria bassiana and Metarhizium anisopliae, to the housefly Musca domestica L.

The pathogenicity of three isolates of Metarhizium anisopliae and two isolates Beauveria bassiana originating from different insect species and localities was tested against housefly larvae. Only one isolate of M. anisopliae (Ma23) and the two isolates of B.