Honeybee proteins in Saudi honeys: A shotgun gel-free proteomic study.

This study investigated the protein content of Acacia and Ziziphus honey samples from the southwestern region of Saudi Arabia following the shotgun gel-free proteomics. Honey proteins were extracted, digested by trypsin and the trypsin digests were separated and characterized using the LC-ESI-QTOF-MS (SCIEX X500R QTOF). The precursor masses of the trypsin digests were used to identify the proteins through searching the mascot spectral database search engine.

leave extracts induce cell death of MCF-7, HepG2, and LS-174T cancer cell lines.

() is a widespread phreatophytic tree, which belongs to the Fabaceae family. The goal of the present study is to investigate the potential anti-cancer effect of leave extracts and to identify its chemical composition. For this purpose, MCF-7 (breast), HepG2 (liver), and LS-174T (colorectal) cancer cell lines were cultivated and incubated with various concentrations of leave extracts, and its impact on cell viability, proliferation, and cell cycle stages was investigated.