Assessment of heavy metal and radioactive element levels in the ovarian follicular fluid of pregnant and non-pregnant Kyrgyz breed mares.

Objective: In the current study, we aimed to ascertain the levels of heavy metals and radioactive elements in the ovarian follicular fluids of pregnant and non-pregnant Kyrgyz mares.

Materials And Methods: To this end, follicular fluids were obtained from 49 Kyrgyz mares aged 3-5 years. The mares were in various stages of pregnancy (Group 1, 27) or were non-pregnant (Group 2, 22).

A comprehensive molecular survey of vector-borne blood parasites in cattle in Kyrgyzstan with a note of the first molecular detection of Anaplasma bovis and Candidatus Anaplasma Camelii.

Vector-borne pathogens continue to increase their impact on the livestock industry worldwide. To protect animals against these pathogens, it is very important to identify the species that cause the disease and understand their prevalence. This study aimed to investigate the presence and prevalence of vector-borne pathogens in apparently healthy cattle in different parts of Kyrgyzstan using molecular diagnostic techniques.

Metrisor: A novel diagnostic method for metritis detection in cattle based on machine learning and sensors.

The Metrisor device has been developed using gas sensors for rapid, highly accurate and effective diagnosis of metritis. 513 cattle uteri were collected from abattoirs and swabs were taken for microbiological testing. The Metrisor device was used to measure intrauterine gases.

Physiological and metabolic responses in Kök-Börü horses: Correlations with game outcomes.

Background: The aim of this study was to examine variations in stress, metabolic, and physiological parameters of horses used in the traditional equestrian team sport of Kök-Börü in relation to winning and losing outcomes.

Material And Methods: To accomplish this, blood samples were taken from horses on four different teams who participated in two separate games, both before and after game. These samples were used to measure levels of cortisol, ACTH, beta-endorphin, adrenaline, noradrenaline, triiodothyronine (T3), and thyroxine (T4) via species-specific commercial ELISA kits.

Genetic analysis of PALB2 gene WD40 domain in canine mammary tumour patients.

Background: DNA repair mechanisms are essential for tumorigenesis and disruption of HR mechanism is an important predisposing factor of human breast cancers (BC). PALB2 is an important part of the HR. There are similarities between canine mammary tumours (CMT) and BCs.

The effects of novel electrical teat dipping on some mastitis parameters in dairy herds.

Electrical teat dipping (ETD) is a novel, patented method developed by the authors to control mastitis in dairy cows. Here we evaluate the efficacy of ETD in reducing the incidence of clinical mastitis and bulk tank milk somatic cell count (BTMSCC) on three dairy farms over 6 months. ETD was applied for morning and evening milking on three farms, while conventional teat dipping (CTD) was applied on the other three farms.

The relationship between Th1/Th2 cytokine polarization and milk composition in the postnatal period in Kyrgyz mares and foals.

Immune compatibility between mare and foal is one of the important topics of reproductive immunology. At this point, although there are many studies on antibodies, there are not many publications on the relationship between the cytokine levels of mare, foal and milk and the effects of milk composition on this relationship. Here we investigate the relationship between Th1/Th2 cytokine balance and milk composition in Kyrgyz mares and foals.

A novel approach in the prevention of mastitis: electrical teat dipping.

Teat dipping is widely used in dairy cattle, especially to protect against contagious mastitis. Here we determine the effect of the device called 'Electrical Teat Dipping' (ETD), which was developed by combining teat dipping application and electrical field stimulation technique on teats. For this purpose, the front teats of 100 Holstein breed milking cows were evaluated in two groups, with ETD being applied once to the left front teat of these cows, and conventional teat dipping (CTD) being applied once to the right front teat, both after milking.

Pulsed-wave Doppler ultrasonographical measurements of supra-mammary lymph nodes for diagnosis of bovine mastitis.

The aim of this study was to investigate the relationship between mastitis and supra-mammary lymph nodes in lactating cows in terms of Pulsed-wave (PW) Doppler ultrasonographical measurements. A total of 102 head cows in lactation period were divided into three groups. The cows in which all mammary lobes were California mastitis test (CMT)-negative (n = 27) formed the 1 group; those with CMT-positive mammary lobe (n = 43) formed the 2 group and the cows with clinical mastitis in at least one mammary lobe (n = 32) formed the 3 group.

Dose related inhibitor effect of enrofloxacin on in vitro feline spontaneous myometrial contractility.

Enrofloxacin is one of the most widely used antibacterial drugs in feline medicine. This study investigated the effects of enrofloxacin on in vitro feline spontaneous myometrial contractility at different sexual stages. Uterine samples of the 20 queen cats at different sexual periods were placed in a tissue bath, and in vitro spontaneous stretch-induced myometrial contractions were recorded for 10 min.

The relationship of asprosin with β-hydroxybutyric acid and postpartum disorders in cows.

The aim of the present study was to determine asprosin levels in cows, the relationship of this hormone with postpartum disorders and β-hydroxybutyric acid, and also the potential of asprosin to be a marker for postpartum diseases. The study was designed as a two-stage trial. In the first stage, blood asprosin and β-hydroxybutyric acid levels of 20 healthy Simmental cows aged 3-4 years were measured at the time of calving, and on days 3, 6, 9, 12 and 15 postpartum.

Expression of the anti-Mullerian hormone, kisspeptin 1, and kisspeptin 1 receptor in polycystic ovary syndrome and controlled ovarian stimulation rat models.

Polycystic ovary syndrome represents a significant cause of female infertility. The aim of this study was to investigate the expression of anti-Mul-lerian hormone (AMH), kisspeptin 1 (KISS-1), and kisspeptin 1 receptor (KISS1r) in rat models of polycystic ovary syndrome (PCOS) and controlled ovarian stimulation (COS). For this purpose, 28 rats were assigned into four groups.

Effects of Ovarian Transplant on Anti-Müllerian Hormone, Kisspeptin-1, and Kisspeptin-1 Receptor Levels in Rats.

Objectives: Information is scarce on levels of kisspeptin-1 and the kisspeptin-1 receptorin females after ovarian transplant. In this study, our aim was to explore serum estradiol, anti-Müllerian hormone, kisspeptin-1, and kisspeptin receptor levels, along with kisspeptin-1-positive cell density, in ovaries from rats after ovarian transplant.

Materials And Methods: For this study, 28 female Sprague Dawley rats were divided into 4 groups, with sham surgery performed on rats in group 1 (control group).

The relationship between mastitis and the B-mode, colour Doppler ultrasonography measurements of supramammary lymph nodes in cows.

This research communication aims to test the hypothesis that B-Mode, colour Doppler ultrasonographic measurements and characteristics can identify mastitis in dairy cows. A total of 102 lactating cows were divided into 3 groups: cows in which all mammary quarters were CMT-negative, cows with CMT-positive mammary quarters and cows with clinical mastitis in at least one quarter. Colour Doppler ultrasonography measurements of the supramammary lymph nodes revealed that distortion-type vascular morphology, the rate of type 4 vascular densities and the incidence of mixed-type vascular distributions were highest in the clinical mastitis group, whereas the frequency of avascularity in supramammary lymph nodes was highest in the CMT-negative group.

A new animal model for uterine torsion and uterine ischemia-reperfusion studies, but not fetal hypoxia studies.

The aim of the present study was to develop a new animal model for use in uterine torsion, uterine ischemia-reperfusion, and fetal hypoxia studies in rats. A total of 14 pregnant rats on their 18th-19th gestational days were used. The animals were randomly divided into two groups: those undergoing the shame operation (group 1), and those in which a 360 uterine torsion was performed using a novel technique, which was corrected 6 hours later (group 2).

The Effects of Post-Mating Administration of Anti-IL-10 and Anti-TGFß on Conception Rates in Mice.

Background: In fertility studies, it has been shown that transforming growth factor β (TGFβ) and interlukin 10 (IL-10) play very important roles in implantation, maternal immune tolerance, placentation and fetal development, and the release beginning of release for fetal and postnatal death. The present study aims to determine the effects of the postmating administration of neutralizing antibodies against IL-10 and TGFβ, which significantly impact pregnancy in females and the conception rates in mice via assessments of blood serum and uterine fluid concentrations of IL-2, IL-4, IL-6, IL-10, IL-17, interferon γ (IFNγ), Tumor necrosis factor α (TNFα), and TGFβ.

Materials And Methods: In this experimental study, 21 BALB/c strain female mice were mated and randomly divided into three groups.

What is the impact of SOCS3, IL-35 and IL17 in immune pathogenesis of recurrent pregnancy loss?

Objective: To investigate the plasma levels of interleukin-4 (IL-4), IL-6, IL-10, tumor necrosis factor-alpha (TNF-alpha), interferon-gamma (IFN-gamma), transforming growth factor-beta (TGF-beta), IL-17, IL-35 and suppressor of cytokine signaling 3 (SOCS3) in the women with history of idiopathic recurrent pregnancy loss (RPL) and in the fertile controls.

Methods: This study was conducted with 60 idiopathic RPL cases and 40 age-matched fertile controls. Mid-follicular plasma levels of IL-17, IFN-gamma, TNF-alpha, TGF-beta, IL-6, IL-4, IL-10, SOCS3 and IL-35 were assayed by an enzyme linked immunosorbent assay.

Antibodies to Schmallenberg virus in domestic livestock in Turkey.

This, partly retrospective study, was designed to determine the seroprevalence of Schmallenberg virus (SBV), a new Orthobunyavirus first reported in Germany in late 2011, in domestic ruminants from the Middle Black Sea, West, and Southeast regions of Turkey. An indirect enzyme-linked immunosorbent assay was used to screen serum samples collected from slaughterhouse animals between 2006 and 2013. The overall seroprevalence was 335/1,362 (24.

Absence of link between abortion and seropositivity of cystic hydatid disease in ewes and female goats in Turkey.

This study was conducted to test whether there is a statistically significant association between seropositivity to cystic echinococcosis and abortion in ewes and female goats from the Turkish provinces of Elazig (east Anatolia), Sanliurfa (south-east Anatolia) and Kayseri (Inner Anatolia) using the enzyme-linked immunosorbent assay (ELISA). A total of 20 of 133 sera (15.1%) from ewes and 5 of 101 sera (4.

The effects of Kisspeptin antibodies on delayed estrus in rats.

In recent years, numerous studies have been conducted on the effects of Kisspeptin/GPR54 system on sexual cycle, which proved that this system regulated gonadotropin release through GnRH. This study aims to determine the effects of hyperimmune serum containing antibodies produced against Kisspeptin on the sexual cycle and GnRH receptors in rat pituitaries. To this end, five Wistar female rats were passively immunised using a hyperimmune serum obtained from two Wistar female rats against Kisspeptin 10.

A study of ovarian autotransplantation without vascular a pedicle in rats.

Purpose: The study aimed to determine effects of FSH applications on follicle survival, development and hormone output and antigenicity of rat ovarian tissue autografts placed at subcutaneous or subperitoneal sites.

Methods: A total of sixteen female rats were used in the study. The animals were divided into three groups.

A new ovarian denervation technique and it's effect on sexual cycle, conception rates and offspring numbers in rats.

Objectives: A novel denervation technique of ovarian was used in rats to investigate its effects on sexual cycle, pregnancy rates and offspring numbers.

Setting And Design: A total of 34 female albino rats were included. Animals were divided into 3 groups.

Use of cauda epididymis extract as immunocontraceptive.

The objective of this study was to evaluate use of a cauda epididymis extract (CEE) as an immunocontraceptive in rats. Twenty-two rats in puberty or 19 rats that gave birth once (primipara) were immunized with intraperitoneal (IP) injection of CEE. Rats in puberty received one or two injections of CEE containing 1.