Molecular and in silico analyses for detection of Shiga toxin-producing Escherichia coli (STEC) and highly pathogenic enterohemorrhagic Escherichia coli (EHEC) using genetic markers located on plasmid, O Island 57 and O Island 71.

Background: Due to the diversity of Shiga toxin-producing Escherichia coli (STEC) isolates, detecting highly pathogenic strains in foodstuffs is challenging. Currently, reference protocols for STEC rely on the molecular detection of eae and the stx1 and/or stx2 genes, followed by the detection of serogroup-specific wzx or wzy genes related to the top 7 serogroups. However, these screening methods do not distinguish between samples in which a STEC possessing both determinants are present and those containing two or more organisms, each containing one of these genes.

Distribution and molecular analysis of Subtilase cytotoxin gene (subAB) variants in Shiga toxin-producing Escherichia coli (STEC) isolated from different sources in Iran.

Subtilase exhibits strong cytotoxicity that was first described in O113:H21 strain in Australia as a plasmid- encoded cytotoxin (subAB1). Subsequently, chromosomal variants including subAB2-1, subAB2-2, and subAB2-3 were described. We aimed to investigate the presence of subAB genes in a collection of Shiga toxin-producing Escherichia coli (STEC) strains (n=101) isolated from different sources in Iran.

Challenges and solutions of medical residency: the example of Iran.

Background: Medical residency constitutes a highly demanding and taxing phase in the professional journey of physicians. Factors such as low income, excessive workloads, and uncertainty regarding their career trajectory can contribute to diminished job satisfaction among residents. Neglecting this issue can have enduring negative effects on the quality and quantity of healthcare services provided.

Enhanced mechanical properties and biocompatibility of hydroxyapatite scaffolds by magnesium and titanium oxides for bone tissue applications.

Significant attention has been devoted to bioactive implants for bone tissue applications, particularly composite scaffolds based on hydroxyapatite (HaP). This study explores the effects of Magnesium and Titanium oxides on the characteristics of HaP-based composite (HMT) scaffolds. The ceramic nanopowders were synthesized using in situ sol-gel, and then the scaffolds were fabricated by gel-casting technique, followed by heat treatment at 1200 °C.

Antimicrobial resistance, β-lactamase genotypes, and plasmid replicon types of Shiga toxin-producing Escherichia coli isolated from different animal hosts.

Aims: The primary objective of this study was to analyze antimicrobial resistance (AMR), with a particular focus on β-lactamase genotypes and plasmid replicon types of Shiga toxin-producing Escherichia coli (STEC) strains originating from various animal hosts.

Methods And Results: A total of 84 STEC strains were isolated from cattle (n = 32), sheep/goats (n = 26), pigeons (n = 20), and wild animals (n = 6) between 2010 and 2018 in various regions of Iran. The Kirby-Bauer susceptibility test and multiple polymerase chain reaction (PCR) panels were employed to elucidate the correlation between AMR and plasmid replicon types in STEC isolates.

Combined dandelion extract and all-trans retinoic acid induces cytotoxicity in human breast cancer cells.

Breast cancer is one of the most prevalent and deadly cancers among women worldwide. Recently, natural compounds have been widely used for the treatment of breast cancer. Present study evaluated antiproliferative and anti-metastasis activities of two natural compounds of dandelion and all-trans-retinoic acid (ATRA) in human MCF-7 and MDA-MB231 breast cancer cells.

Training needs assessment of hospital CEOs in a developing country: the example of Iran.

Background: It is essential to identify the necessary competencies of hospital CEOs in order to improve the quality and efficiency of services they provide. Expert leadership skills and competencies can have a significant impact on the success of an organization, benefiting both patients and staff. This study aimed to assess the competencies and training needs of hospital CEOs in Iran public hospitals.

Global prevalence of nosocomial infection: A systematic review and meta-analysis.

Objectives: Hospital-acquired infections (HAIs) are significant problems as public health issues which need attention. Such infections are significant problems for society and healthcare organizations. This study aimed to carry out a systematic review and a meta-analysis to analyze the prevalence of HAIs globally.

A systemmatic literature review on indirect costs of women with breast cancer.

Introduction: The rising incidence of breast cancer places a financial burden on national health services and economies. The objective of this review is to present a detailed analysis of the research and literature on indirect costs of breast cancer.

Methods: English literature databases from 2000 to 2020 were searched to find studies related to the objective of the present review.

Integrated and People-centred Hospital toward Universal Health Coverage in WHO- European Countries.

Ensuring integrated people-centred health services (IPCHS) that offer universal access, social equity, and financial protection within a primary health care method is important toward universal health coverage and health sustainable development goals. Hospitals are part of this ambitious agenda. The purpose is to review the health system and to list and summarize hospital interventions.

Designing and Evaluating a Mobile-based Self-care Application for Patients with Gastrointestinal Cancer to Manage Chemotherapy Side Effects.

Controlling and managing the side effects of chemotherapy is one of the most serious challenges that patients with gastrointestinal cancer encounter. A promising technique to overcome these challenges is using informative mobile-based applications. The aim of this study was to design and evaluate a mobile-based application to help patients with gastrointestinal cancer to manage the possible side effects caused by chemotherapy.

Aflatoxin M1 in distributed milks in northwestern Iran: occurrence, seasonal variation, and risk assessment.

Aflatoxin is one of the dangerous fungal toxins that is produced in the presence of humidity and heat and lack of proper storage conditions and is considered as a dangerous substance threatening human health. The work aimed to determination of aflatoxin M1 (AFM) level in raw, pasteurized, and sterilized milks offered in the study area and to evaluate the risk of its consumption. In this descriptive cross-sectional study, 60 samples of milk types supplied in the region were collected during two series of sampling (cold and hot seasons) and analyzed by ELISA method.

High prevalence of clonally related multiple resistant Salmonella Infantis carrying class 1 integrons in broiler farms.

The poultry industry in Iran is the main supplier of protein in the food chain. In the present study, we showed the importance of the possible dissemination of clonally related multiple drug resistant (MDR) Salmonella Infantis in broiler farms in Iran. In total, 156 fecal samples belonging to 23 poultry farms in Razavi Khorasan province, northeast of Iran, were examined for the presence of Salmonella serovars.

The Impact of Inflation and Its Uncertainty on Pharmaceutical Prices: Evidence from Iran.

Pharmaceutical productions are recognized as an essential commodity in the economical literature; therefore, an increase in their prices leads to an increase in the household budget. Currently, about 15-20% of the entire health expenditure in Iran is allocated to the pharmaceutical sector. This study aimed to investigate the effect of inflation and its uncertainty on inflation in pharmaceutical prices in Iran.

Glutamine supplementation can reduce some atherosclerosis markers after exhaustive exercise in young healthy males.

Objectives: Glutamine can be beneficial to athletes for its antiinflammatory and antioxidant effects. The present study was designed to investigate the effect of glutamine supplementation on some atherosclerosis markers after exhaustive exercise in young healthy males.

Methods: In an intervention study, 30 healthy males (case = 15 and control = 15) were randomly assigned into two groups.

Feasibility and Therapeutic Potential of Peptide Receptor Radionuclide Therapy for High-Grade Gliomas.

Purpose: This pilot study tested the principle that 177Lu-DOTATATE may be applied to patients with high-grade gliomas (HGGs) that are either inoperable or refractory to the standard conventional treatments and also assessed whether this approach could be a viable therapeutic plan in this dilemma.

Methods: In this prospective study, 16 subjects experiencing HGGs that were either inoperable or refractory to the standard conventional treatments were included. All the patients checked for somatostatin receptor expression on the tumors.

Comparison of the effects of zinc oxide and zinc oxide nanoparticles on the expression of hepcidin gene in rat liver.

Objectives: Nanoparticles have special properties, such as increased intestinal absorption, permeability, and so on. Zinc oxide (ZnO) nanoparticles have medical applications such as using in drug production. Studies of ZnO nanoparticles have shown the role of these particles in reducing or increasing the genes expression.

Training needs assessment of nursing managers for achieving university health coverage.

Background: University health coverage gives everyone accesses to health services focused on the most important causes of illness and death and ensures that the quality of services is high enough to improve the health of those receiving services. Nurses as the largest group of staff in the health system play an important role in achieving UHC. This study aimed to determine the training needs of nursing managers in universities of medical sciences of Iran in achieving UHC.

Effects of conjugated linoleic acid supplementation on serum levels of interleukin-6 and sirtuin 1 in COPD patients.

Objective: Chronic obstructive pulmonary disease (COPD) is characterized by systemic inflammation and accelerated inflammaging of the lungs. Some studies showed that conjugated linoleic acid (CLA) has anti-inflammatory effects. The aim of the present study was to evaluate the effect of CLA supplementation on serum levels of interleukin (IL)-6 and sirtuin1 (SIRT1) in patients with COPD.

Comparison between electrochemical and photoelectrochemical detection of dopamine based on titania-ceria-graphene quantum dots nanocomposite.

In this study, titania-ceria-graphene quantum dot (TC-GQD) nanocomposite was synthesized by hydrothermal method for the first time. The prepared nanomaterials were characterized by XRD, FTIR dynamic light scattering (DLS), FESEM, HRTEM, and EDX spectroscopy along with elemental mapping. The synergistic effect of the nanocomposite components was studied by diffuse reflectance spectroscopy (DRS) and electrical conductivity meter.

Copper and Zinc Levels in Myelodysplastic Syndrome Patients versus Healthy Subjects.

Background: Myelodysplastic syndrome (MDS) is a heterogeneous hematological disease and certain serum factors are assumed to be involved in its pathogenesis and progression. Given this, our aim was to comparatively investigate the copper, zinc, and iron levels in MDS patients and healthy individuals.

Methods: This case-control study was conducted on 31 patients with MDS (according to the WHO criteria after investigating laboratory tests such as peripheral blood smear and bone marrow aspiration) attending Bahonar Hospital, Kerman, Iran, and 31 healthy subjects from 2016 to 2018.

The Effect of Glutamine Supplementation on Oxidative Stress and Matrix Metalloproteinase 2 and 9 After Exhaustive Exercise.

Background: Glutamine is the most abundant amino acid in plasma and skeletal muscles and an important fuel for immune system cells. It has beneficial anti-inflammatory and antioxidant properties which may be considered as a potentially useful supplement for athletes. The present study was conducted to investigate the effect of glutamine supplementation on oxidative stress and matrix metalloproteinase 2 and 9 after exhaustive exercise in young healthy males.