A novel biosensing strategy for identification of three important bacteria causing meningitis.

Bacterial meningitis is an acute infection which requires rapid diagnosis and treatment due to the high mortality and serious consequences of the disease. The purpose of this study was to design a homemade multiplex PCR and a novel fluorescence biosensor on chip (FBC) to detect three important agents of meningitis including Streptococcus pneumoniae (S. pneumoniae), Neisseria meningitidis (N.

The effects of citrus flavonoids supplementation on endothelial function: A systematic review and dose-response meta-analysis of randomized clinical trials.

The present systematic review and dose-response meta-analysis was conducted to synthesize existing data from randomized clinical trials (RCTs) concerning the impact of citrus flavonoids supplementation (CFS) on endothelial function. Relevant RCTs were identified through comprehensive searches of the PubMed, ISI Web of Science, and Scopus databases up to May 30, 2023. Weighted mean differences and their corresponding 95% confidence intervals (CI) were pooled utilizing a random-effects model.

The induction of tau aggregation is restricted by sulfamethoxazole and provides new information regarding the use of the drug.

The aggregation of tau protein in the form of paired helical filament (PHF) leads to the breakdown of microtubule structure and the development of neurodegenerative disorders, such as Alzheimer's disease. Therefore, inhibiting tau protein aggregation is a potential strategy for preventing the progression of these disorders. In this study, sulfamethoxazole (SMZ), an antibiotic that easily crosses the blood-brain barrier and interacts with tau protein, was tested for its ability to inhibit tau aggregation .

Corrigendum to "Introducing an interesting and novel strategy based on exploiting first-order advantage from spectrofluorimetric data for monitoring three toxic metals in living cells" [Toxicol. Rep. 9 (2022) 647-655].

[This corrects the article DOI: 10.1016/j.toxrep.

Synthesis, characterization and hypolipidemic effects of urazine derivatives on rat: Study of molecular modeling and enzyme inhibition.

The prevalence of hyperlipidemia has increased dramatically worldwide. It is a major public health threat, characterized by the presence of an abnormal lipid profile, primarily with elevated serum total cholesterol (TC), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL) levels, and reduced high-density lipoprotein (HDL) level. Genetic factors, dietary and lifestyle habits play important roles in hyperlipidemia.

Ultrasensitive biosensing of thiram based on detection of the DNA damage induced by thiram: Application to investigation of protective effects of extra virgin olive oil against DNA damage.

In this work, a novel electrochemical biosensor was fabricated based on modification of a glassy carbon electrode (GCE) with nafion-DNA/gold nanoparticles/poly-ethylenedioxy pyrrole/multi-walled carbon nanotubes-ionic liquid (NF-DNA/Au NPs/PEDOP/MWCNTs-IL/GCE) with the aim of amperometric detection of the DNA damage induced by thiram (TH). By incubation of the biosensor with the TH, the TH was intercalated within DNA, and the exposed DNA released negative charges at the surface of the biosensor which repelled the probe molecules and caused the amperometric response of the biosensor to be decreased. Protective effects of extra virgin olive oil (EVOO) on the DNA damage induced by the TH were investigated by recording amperometric responses of the biosensor in the presence of EVOO, and the results confirmed that the response of the biosensor didn't change to confirm the protective effects of the EVOO on preventing the DNA damage induced by the TH.

Chemometrics-assisted electrochemical biosensing of cholesterol as the sole precursor of steroids by a novel electrochemical biosensor.

This project was performed with the aims of increasing the sensitivity of differential pulse voltammetry (DPV) which itself is a sensitive electroanalytical technique, and also to compare the area under peak (univariate calibration), height of peak (univariate calibration) and whole of vector (multivariate calibration) for calibration purposes. These topics were investigated by fabrication of a novel electrochemical biosensor for determination of cholesterol (CHO). The procedure used in this project was based on the synthesis of molecularly imprinted polymers (MIPs) to the preconcentration of CHO and its biosensing by a rotating glassy carbon electrode (GCE) modified by co-immobilization of cholesterol oxidase (CO), cholesterol esterase (CE) and horseradish peroxidase (HP) onto multiwalled carbon nanotubes-ionic liquid (COCEHP/MWCNTs-IL/GCE).

Sensitive and selective simultaneous biosensing of nandrolone and testosterone as two anabolic steroids by a novel biosensor assisted by second-order calibration.

Recently, our research group have focused on an interesting project in which a novel dual template molecularly imprinted (DTMIP) biosensor was fabricated and assisted by second-order differential pulse voltammetric (DPV) data for simultaneous determination of nandrolone decanoate (ND) and testosterone decanoate (TS). An indium tin oxide (ITO) was modified with multiwalled carbon nanotubes-graphene-ionic liquid (MWCNT-Gr-IL) and then, the fullerene C was casted onto the surface of MWCNT-Gr-IL/ITO and electrochemically reduced. Finally, DTMIPs were electrosynthesized by electropolymerization of 4-aminobenzoic acid (ABA) as monomer with ND and TS as template molecules to obtain the final structure of the biosensor (DTMIP/C/MWCNT-Gr-IL/ITO).

Simultaneous estimation of rates of DNA damage induced by three important chemotherapy drugs by a novel electrochemical biosensor assisted by chemometric multivariate calibration methods.

In this work, a novel electrochemical biosensor assisted by multivariate calibration methods was developed for simultaneous estimation of rates of DNA damage induced by doxorubicin (DX), daunorubicin (DR) and idarubicin (ID), and also to simultaneous determination of the drugs. A glassy carbon electrode was efficiently modified and used as the biosensing platform. Binding and interactions of DX, DR and ID with DNA were modeled by molecular docking methods, and theoretical information was completed by experimental results.

Introducing an interesting and novel strategy based on exploiting first-order advantage from spectrofluorimetric data for monitoring three toxic metals in living cells.

In this work, we did our best to develop a novel and interesting analytical method based on coupling of spectrofluorimetry with first-order multivariate calibration techniques for simultaneous determination of lead (Pd), zinc (Zn) and cadmium (Cd) in HeLa cells. To achieve this goal, quenching of the emission of graphene (GR) was individually investigated in the presence of Pb, Zn and Cd and then, according to the linear ranges obtained from individual calibration graphs, a multivariate calibration model was developed based on modeling of the quenching of the emission of GR in the presence of the mixtures of Pb, Zn and Cd. First-order multivariate calibration models were constructed by partial least squares (PLS), principal component regression (PCR), orthogonal signal correction-PLS (OSC-PLS), continuum power regression (CPR), robust continuum regression (RCR) and partial robust M-regression (PRM) and their performances were evaluated and statistically compared.

Effects of backpack-induced fatigue on gait ground reaction force characteristics in primary school children with flat-foot deformity.

This study aimed to assess the effects of backpack carriage in different weights and muscle fatigue on the GRF components in primary school children with flat-foot deformity. The GRF components from 42 primary school children (21 with low arch, and 21 with normal) were collected before and after backpack-induced fatigue protocol during shod walking with backpacks in weight 7.5, 10, 12.

Evaluation of Antimicrobial and Wound Healing Effects of Gold Nanoparticles Containing (L.) Aqueous Extract.

. Wound healing is a complex process of replacing devitalized cellular structures and tissues with healthy cells and tissue. Nanotechnology has been increasingly proposed as a novel platform to treat wounds and skin regeneration.

Resolving interactions of miglitol with normal and glycated human serum albumin by multivariate methods.

This article reports results of one of our projects related to the investigation of interactions of miglitol (MIG) with normal human serum albumin (HSA) and glycated HSA (GHSA) with the help of recording spectroscopic and electrochemical data. The experimental data were analyzed by conventional and chemometric methods to extract useful information for comprehensive justifications of the interactions of the MIG with HSA and GHSA. Hard- and soft-modeling chemometric methods were used to extract quantitative and qualitative information.

Chemometrics in investigation of small molecule-biomacromolecule interactions: A review.

Chemometrics is chemical discipline in which mathematical and statistical methods are coupled with chemical data to extract useful information which cannot be extracted by the use of conventional methods. When experimental techniques are assisted by chemometric methods, very interesting studies will be performed which enable us to obtain valuable information about the system under our study. Chemico-biological interactions are very useful studies which are performed to obtain information about binding of small molecules with biological macromolecules.

Chemometric modeling of the electrochemical data to investigate proline cis/trans isomeration effect on aggregation of Tau protein.

Tau protein (Tau) is a proline-rich protein and in this work, we have developed a very interesting strategy based on combination of electrochemistry with chemometric methods to investigate proline cis/trans isomeration effect on the Tau aggregation. To achieve this goal, the proline residues at RTPPK motif have been replaced by alanine to generate RTPAK, RTAPK and RTAAK mutants of the Tau. Then, cyclic voltammetric (CV) responses of the Tau and RTPAK, RTAPK and RTAAK as its mutants in the presence of heparin (HEP) as an anionic inducing agent which could trigger aggregation of the Tau were recorded at physiological conditions every hour during 12 h.

Coupling of digital image processing and three-way calibration to assist a paper-based sensor for determination of nitrite in food samples.

In this work, a novel and very interesting analytical methodology based on coupling of digital image processing and three-way calibration has been developed for determination of nitrite in food samples. Nitrite in contact with Griess reagent is able to produce a red-colored azo dye whose color intensity is correlated with nitrite concentration and here, a piece of Whatman filter paper impregnated with Griess reagent was used as the platform of the sensor and a SONY Xperia Z5 cell phone was used for image capturing from the sensor surface. To generate second-order data, the -number of the camera's sensor was changed as an instrumental parameter.

An elegant technology for ultrasensitive impedimetric and voltammetric determination of cholestanol based on a novel molecularly imprinted electrochemical sensor.

In this work, a novel molecularly imprinted electrochemical sensor (MIES) has been fabricated based on electropolymerization of a molecularly imprinted polymer (MIP) onto a glassy carbon electrode (GCE) modified with gold-palladium alloy nanoparticles (AuPd NPs)/polydopamine film (PDA)/multiwalled carbon nanotubes-chitosan-ionic liquid (MWCNTs-CS-IL) for voltammetric and impedimetric determination of cholestanol (CHO). Modifications applied to the bare GCE formed an excellent biocompatible composite film which was able to selectively detect CHO molecules. Modifications applied to the bare GCE were characterized by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (SEM).

Developing an elegant and integrated electrochemical-theoretical approach for detection of DNA damage induced by 4-nonylphenol.

In this work, a novel biosensor was fabricated for detection of DNA damage induced by 4-nonylphenol (NP) and also determination of NP. To achieve this goal, a glassy carbon electrode (GCE) was modified with chitosan (Chit), gold nanoparticles (Au NPs) and DNA-multiwalled carbon nanotubes (DNA-MWCNTs). Then, the DNA-MWCNTs/Au NPs/Chit/GCE was incubated with methylene blue (MB) to obtain MB-DNA-MWCNTs/Au NPs/Chit/GCE in which MB was used as the redox indicator.

Pyridine-2,3-dicarboxylate, quinolinic acid, induces 1N4R Tau amyloid aggregation in vitro: Another evidence for the detrimental effect of the inescapable endogenous neurotoxin.

Quinolinic acid (QA) known as a neuro-active metabolite associated with the kynurenine pathway. At high concentrations, QA is often involved in the initiation and development of several human neurologic diseases, like Alzheimer's disease. Because of the QA action as the NMDA receptor, it is considered as a potent excitotoxin in vivo.

Matrix augmentation as an efficient method for resolving interaction of bromocriptine with human serum albumin: trouble shooting and simultaneous resolution.

This work reports the results of an interesting study related to the investigation of interactions of bromocriptine (BCP) with human serum albumin (HSA) by mathematicall modelling of voltammetric and spectroscopic data into an augmented data matrix and its resolution by multivariate curve resolution-alternating least squares (MCR-ALS). The quality of the results obtained by MCR-ALS was examined by MCR-BANDS and its outputs confirmed the absence of rotational ambiguities in the MCR-ALS results. BCP-HSA interactions were also modeled by molecular docking methods to verify the results obtained from experimental sections and fortunately, they were compatible.

Chemometrical-electrochemical investigation for comparing inhibitory effects of quercetin and its sulfonamide derivative on human carbonic anhydrase II: Theoretical and experimental evidence.

This paper reports results of a valuable study on investigation of inhibitory effects of the sulfonamide derivative of quercetin (QD) on human carbonic anhydrase II (CA-II) by electrochemical and chemometrical approaches. To achieve this goal, a glassy carbon electrode (GCE) was chosen as the sensing platform and different electrochemical techniques such as cyclic voltammetry (CV), differential pulse voltammetry (DPV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) were used to investigate and comparing inhibitory effects of quercetin (Q) and QD on CA-II. By the use of EQUISPEC, SPECFIT, SQUAD and REACTLAB as efficient hard-modeling algorithms, bindings of Q and QD with CA-II were investigated and the results confirmed that the QD inhibited the CA-II stronger than Q suggesting a highly relevant role of QD's-SONH group in inhibiting activity and also was confirmed by docking studies.

Chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal properties of ethanolic extract of Allium Saralicum R.M. Fritsch leaves rich in linolenic acid, methyl ester.

The purpose of the recent research was to assess the chemical characterization and antioxidant, cytotoxic, antibacterial, and antifungal effects of Allium Saralicum R.M. Fritsch leaves.

Fabrication of a novel and ultrasensitive label-free electrochemical aptasensor for detection of biomarker prostate specific antigen.

In this study, a novel and efficient aptasensor based on immobilization of thiol terminated prostate specific antigen (PSA) binding DNA aptamer onto Au nanoparticles/fullerene C-chitosan-ionic liquid/multiwalled carbon nanotubes/screen printed carbon electrode has been fabricated for ultrasensitive aptasensing of biomarker PSA. Formation of PSA-aptamer complex caused a variation in electrochemical impedance spectroscopic (EIS) and differential pulse voltammetric (DPV) responses of the aptasensor which enabled us to aptasensing of the PSA by EIS and DPV methods. Morphology and electrochemical properties of the fabricated aptasensor were examined by scanning electron microscopy (SEM), cyclic voltammetry (CV) and EIS.

MATLAB in electrochemistry: A review.

MATLAB (MATrix LABoratory) is a multi-paradigm numerical computing environment and fourth-generation programming language. MATLAB allows matrix manipulations, plotting of functions and data, implementation of algorithms, creation of user interfaces and interfacing with programs written in other languages, including C, C, Java, Fortran and Python. Electrochemistry is a branch of chemistry that studies the relationship between electricity, as a measurable and quantitative phenomenon, and identifiable chemical change, with either electricity considered an outcome of a particular chemical change or vice versa.