Campylobacter jejuni induces diverse kinetics and profiles of cytokine genes in INT-407 cells.

Objective: To examine the kinetic ability of embryonic human epithelial INT-407 cells to express messenger ribonucleic acid mRNA for various cytokines and chemokines in response to Campylobacter jejuni C. jejuni stimulation.

Methods: In an experimental single-blind study, cultured embryonic human epithelial INT-407 cells were treated with different concentrations of viable C.

Multiplex PCR for direct identification of Campylobacter spp. in human and chicken stools.

Differentiation between Campylobacter jejuni and Campylobacter coli is problematic in clinical specimens due to fastidious growth requirements and limited biochemical tests. This study describes a rapid, multiplex PCR protocol for the direct detection and differentiation of C. jejuni and C.

Human papillomavirus infection among women attending health facilities in the Kingdom of Bahrain.

Objective: To investigate the occurrence of human papillomavirus (HPV) infection and the associated risk factors in Bahrain's female population.

Methods: This study was carried out between March to December 2004, which includes cervical scrapings for Pap smear and HPV-DNA testing using polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis, obtained from 100 women attending the Gynecology Clinic at Salmaniya Medical Center and Sheikh Sabah Health Center in the Kingdom of Bahrain. We distributed questionnaires that include the sociodemographic data as well as information on risk factors such as smoking, parity, and the contraceptive used.