Predictive factors of successful microdissection testicular sperm extraction in patients with presumed sertoli cell-only syndrome.

Background: To evaluate predictive factors of successful microdissection-testicular sperm extraction (MD-TESE) in patients with presumed Sertoli cell-only syndrome (SCOS).

Materials And Methods: In this retrospective analysis, 874 men with non-obstructive azoospermia (NOA), among whom 148 individuals with diagnosis of SCOS in prior biopsy, underwent MD-TESE at Department of Andrology, Royan Institute, Tehran, Iran. The predictive values of follicle stimulating hormone (FSH), luteinizing hormone (LH), and testosterone (T) levels, testicular volume, as well as male age for retrieving testicular sperm by MD-TESE were analyzed by multiple logistic regression analysis.

Achieving high survival rate following cryopreservation after isolation of prepubertal mouse spermatogonial cells.

Purpose: Isolating spermatogonia cells with high purity and viability and achieving better survival rate following cryopreservation

Methods: Isolating the cells by Magnetic Activating Cell Sorting (MACS) method using anti CD49f (alpha6 integrin) antibody and Dynabeads and freezing in DMSO-based freezing mediums containing three different FBS concentrations of 50%, 60% and 70%.

Results: The mean (+/-SD) purity of the isolated cells was 92.52+/-3.

Topiramate prevents ecstasy consumption: a case report.

The last decade has witnessed a development in the phenomenon 'ecstasy'. Several substances, with more or less the same effects, are grouped together by the term ecstasy, the best-known one being 3,4-methylenedioxymethamphetamine (MDMA). The psychopathological consequences of MDMA in humans are relatively poorly understood.