Biochar application affected biochemical properties, yield and nutrient content of safflower under water stress.

A two-year field trial was set up to investigate the effects of applying 3 tons ha of wheat (3WB) and cotton biochar (3CB) alone or in combination with chemical nitrogen (N) and phosphorus (P) fertilizers on biochemical properties, yield and nutrient content of safflower under normal irrigation and water stress (irrigation cut-off at flowering stage) conditions. The total water applied in the chemical treatments [150 kg ha N + 50 kg ha P (100% of the recommended dose) and 112.5N + 37.

In silico identification of miRNAs and their target genes and analysis of gene co-expression network in saffron ( L. stigma.

As an aromatic and colorful plant of substantive taste, saffron ( L.) owes such properties of matter to growing class of the secondary metabolites derived from the carotenoids, apocarotenoids. Regarding the critical role of microRNAs in secondary metabolic synthesis and the limited number of identified miRNAs in , on the other hand, one may see the point how the characterization of miRNAs along with the corresponding target genes in might expand our perspectives on the roles of miRNAs in carotenoid/apocarotenoid biosynthetic pathway.

Genome-wide transcriptomic analysis of the effects of sub-ambient atmospheric oxygen and elevated atmospheric carbon dioxide levels on gametophytes of the moss, Physcomitrella patens.

It is widely accepted that atmospheric O2 has played a key role in the development of life on Earth, as evident from the coincidence between the rise of atmospheric O2 concentrations in the Precambrian and biological evolution. Additionally, it has also been suggested that low atmospheric O2 is one of the major drivers for at least two of the five mass-extinction events in the Phanerozoic. At the molecular level, our understanding of the responses of plants to sub-ambient O2 concentrations is largely confined to studies of the responses of underground organs, e.

Evaluation of the use of the polyubiquitin genes, Ubi4 and Ubi10 as reference genes for expression studies in Brachypodium distachyon.

Background: Brachypodium distachyon is emerging as the model plant for temperate grass research and the genome of the community line Bd21 has been sequenced. Additionally, techniques have been developed for Agrobacterium-mediated transformation for the generation of T-DNA insertional lines. Recently, it was reported that expression of the polyubiquitin genes, Ubi4 and Ubi10 are stable in different tissues and growth hormone-treated plant samples, leading to the conclusion that both Ubi4 and Ubi10 are good reference genes for normalization of gene expression data using real-time, quantitative PCR (qPCR).