Acclimatizing and training freely viewing marmosets for behavioral and electrophysiological experiments in oculomotor tasks.

The marmoset is a small-bodied primate with behavioral capacities and brain structures comparable to macaque monkeys and humans. Its amenability to modern biotechnological techniques like optogenetics, chemogenetics, and generation of transgenic primates have attracted neuroscientists' attention to use it as a model in neuroscience. In the past decade, several laboratories have been developing and refining tools and techniques for performing behavioral and electrophysiological experiments in this new model.

Nicotinamide adenine dinucleotide hydrogen regeneration in a microbial electrosynthesis system by Enterobacter aerogenes.

Costly cofactors such as nicotinamide adenine dinucleotide hydrogen (NADH) are essential to have high activity in many redox enzymatic processes. Cofactor regeneration methods have been suggested to improve the economic aspects of the system. Here, we introduce a microbial electrosynthesis process to regenerate NADH in a two-chamber set-up with Enterobacter aerogenes biofilm as the bio-cathode.

Spatially precise visual gain control mediated by a cholinergic circuit in the midbrain attention network.

A primary function of the midbrain stimulus selection network is to compute the highest-priority location for attention and gaze. Here we report the contribution of a specific cholinergic circuit to this computation. We functionally disconnected the tegmental cholinergic nucleus isthmi pars parvocellularis (Ipc) from the optic tectum (OT) in barn owls by reversibly blocking excitatory transmission in the Ipc.

Rules of competitive stimulus selection in a cholinergic isthmic nucleus of the owl midbrain.

In a natural scene, multiple stimuli compete for the control of gaze direction and attention. The nucleus isthmi pars parvocellularis (Ipc) is a cholinergic, midbrain nucleus that is reciprocally interconnected to the optic tectum, a structure known to be involved in the control of gaze and attention. Previous research has shown that the responses of many Ipc units to a visual stimulus presented inside the classical receptive field (RF) can be powerfully inhibited when the strength of a distant, competing stimulus becomes the stronger stimulus.

Signaling of the strongest stimulus in the owl optic tectum.

Essential to the selection of the next target for gaze or attention is the ability to compare the strengths of multiple competing stimuli (bottom-up information) and to signal the strongest one. Although the optic tectum (OT) has been causally implicated in stimulus selection, how it computes the strongest stimulus is unknown. Here, we demonstrate that OT neurons in the barn owl systematically encode the relative strengths of simultaneously occurring stimuli independently of sensory modality.

Neural correlates of binaural masking level difference in the inferior colliculus of the barn owl (Tyto alba).

Humans and animals are able to detect signals in noisy environments. Detection improves when the noise and the signal have different interaural phase relationships. The resulting improvement in detection threshold is called the binaural masking level difference.

Stimulus-driven competition in a cholinergic midbrain nucleus.

The mechanisms by which the brain selects a particular stimulus as the next target for gaze are poorly understood. A cholinergic nucleus in the owl's midbrain exhibits functional properties that suggest its role in bottom-up stimulus selection. Neurons in the nucleus isthmi pars parvocellularis (Ipc) responded to wide ranges of visual and auditory features, but they were not tuned to particular values of those features.

Global inhibition and stimulus competition in the owl optic tectum.

Stimulus selection for gaze and spatial attention involves competition among stimuli across sensory modalities and across all of space. We demonstrate that such cross-modal, global competition takes place in the intermediate and deep layers of the optic tectum, a structure known to be involved in gaze control and attention. A variety of either visual or auditory stimuli located anywhere outside of a neuron's receptive field (RF) were shown to suppress or completely eliminate responses to a visual stimulus located inside the RF in nitrous oxide sedated owls.

Distribution of interaural time difference in the barn owl's inferior colliculus in the low- and high-frequency ranges.

Interaural time differences are an important cue for azimuthal sound localization. It is still unclear whether the same neuronal mechanisms underlie the representation in the brain of interaural time difference in different vertebrates and whether these mechanisms are driven by common constraints, such as optimal coding. Current sound localization models may be discriminated by studying the spectral distribution of response peaks in tuning curves that measure the sensitivity to interaural time difference.

Genomic and nongenomic effects of intrahippocampal microinjection of testosterone on long-term memory in male adult rats.

In addition to their well-known genomic effects via intracellular receptors, androgens rapidly alter neuronal excitability through a nongenomic pathway. The nongenomic effect of testosterone, as the main androgen, apart from its traditional effects, was assessed in one of the fundamental centers of learning and memory, the hippocampus, on long-term memory (LTM) in passive avoidance conditioning. Different doses of testosterone enanthate (T) or testosterone-BSA (T-BSA) bilaterally were injected into the CA1 region of the hippocampus 15 min before shock delivery (1 mA during 5 s) in a two-compartment passive avoidance apparatus.